Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development

Author(s):Show less -

Moraes, Angela M. ^[1] ; Jorge, Soraia A. C. ^[2] ; Astray, Renato M. ^[2] ; Suazo, Claudio A. T. ^[3] ; Calderon Riquelme, Camilo E. ^[3] ; Augusto, Elisabeth F. P. ^[4] ; Tonso, Aldo ^[5] ; Pamboukian, Marilena M. ^[5] ; Piccoli, Rosane A. M. ^[4] ; Barral, Manuel F. ^[4] ; Pereira, Carlos A. ^{[5, 2]}

Affiliation

^[1] Univ Estadual Campinas, Fac Engn Quim, Dept Engn Mat & Bioproc, BR-13083852 Campinas, SP - Brazil

^[2] Inst Butantan, Lab Imunol Viral, BR-05533900 Sao Paulo - Brazil

^[3] Univ Fed Sao Carlos, Dept Engn Quim, BR-13560 Sao Carlos, SP - Brazil

^[4] Inst Pesquisas Tecnol Estado Sao Paulo SA, Ctr Tecnol Proc & Prod, Lab Bietecnol Ind, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Escola Politecn, Lab Celulas Anim, Dept Engn Quim, Sao Paulo - Brazil

• Document type: Review article
• Source: BIOTECHNOLOGY ADVANCES; v. 30, n. 3, p. 613-628, MAY-JUN 2012.
• Web of Science Citations: 33

Abstract

In the present review we discuss strategies that have been used for heterologous gene expression in Drosophila melanogaster Schneider 2 (S2) cells using plasmid vectors. Since the growth of S2 cells is not dependent on anchorage to solid substrates, these cells can be easily cultured in suspension in large volumes. The factors that most affect the growth and gene expression of S2 cells, namely cell line, cell passage, inoculum concentration, culture medium, temperature, dissolved oxygen concentration, pH, hydrodynamic forces and toxic metabolites, are discussed by comparison with other insect and mammalian cells. Gene expression, cell metabolism, culture medium formulation and parameters involved in cellular respiration are particularly emphasized. The experience of the authors with the successful expression of a biologically functional protein, the rabies virus glycoprotein (RVGP), by recombinant S2 cells is presented in the topics covered. (C) 2011 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 02/09482-3 - Heterologus gene expression in dipteran cells: molecular biology and bioprocess engineering
• Grantee: Carlos Augusto Pereira
• Support Opportunities: Research Projects - Thematic Grants