Low-Level Laser Therapy Influences Mouse Odontoblast-Like Cell Response in Vitro

Author(s)

Pereira, Luciana Batista ^[1] ; Chimello, Daniela Thomazatti ^[1] ; Wimmers Ferreira, Maidy Rehder ^[1] ; Bachmann, Luciano ^[2] ; Rosa, Adalberto Luiz ^[3] ; Bombonato-Prado, Karina Fittipaldi ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, Dept Morphol Stomatol & Physiol, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Phys, Fac Philosophy Sci & Languages Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral Maxillofacial Surg & Periodontol, BR-14040904 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Photomedicine and Laser Surgery; v. 30, n. 4, p. 206-213, APR 2012.
• Web of Science Citations: 13

Abstract

Objective: The purpose of this study was to analyze the influence of two different irradiation times with 85mW/cm(2) 830nm laser on the behavior of mouse odontoblast-like cells. Background data: The use of low-level laser therapy (LLLT) to stimulate pulp tissue is a reality, but few reports relate odontoblastic responses to irradiation in in vitro models. Methods: Odontoblast-like cells (MDPC-23) were cultivated and divided into three groups: control/nonirradiated (group 1); or irradiated with 85mW/cm(2), 830nm laser for 10 sec (0.8 J/cm(2)) (group 2); or for 50 sec (4.2 J/cm(2)) (group 3) with a wavelength of 830 nm. After 3, 7, and 10 days, it was analyzed: growth curve and cell viability, total protein content, alkaline phosphatase (ALP) activity, calcified nodules detection and quantification, collagen immunolocalization, vascular endothelial growth factor (VEGF) expression, and real-time polymerase chain reaction (PCR) for DMP1 gene. Data were analyzed by Kruskall-Wallis test (alpha = 0.05). Results: Cell growth was smaller in group 2 (p < 0.01), whereas viability was similar in all groups and at all periods. Total protein content and ALP activity increased on the 10th day with 0.8 J/cm(2) (p < 0.01), as well as the detection and quantification of mineralization nodules (p < 0.05), collagen, and VEGF expression (p < 0.01). The expression of DMP1 increased in all groups (p < 0.05) compared with control at 3 days, except for 0.8 J/cm(2) at 3 days and control at 10 days. Conclusions: LLLT influenced the behavior of odontoblast-like cells; the shorter time/smallest energy density promoted the expression of odontoblastic phenotype in a more significant way. (AU)

• FAPESP's process: 06/06313-7 - In vitro evaluation of biostimulation of mouse odontoblastic cells (MDPC-23) with low level laser therapy (GAAlAs)
• Grantee: Karina Fittipaldi Bombonato Prado
• Support type: Regular Research Grants