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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

RHD alleles in Brazilian blood donors with weak D or D-negative phenotypes

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Author(s):
Cruz, B. R. [1] ; Chiba, A. K. [1] ; Moritz, E. [1] ; Bordin, J. O. [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Disciplina Hematol & Hemoterapia, BR-04023092 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Transfusion Medicine; v. 22, n. 2, p. 84-89, APR 2012.
Web of Science Citations: 23
Abstract

The RHD gene is highly polymorphic and the existence of a large number of alleles results in RhD variant phenotypes. RHD genotyping has been used to distinguish normal D antigen from D variants due to limitations of serologic methods. The purpose of this study was to determine the phenotypic frequency of RhD and RhCE antigens and to investigate the RHD alleles present in samples with the weak D or D- phenotypes from Brazilian blood donors. A total of 2007 donors were phenotyped for D, C, c, E and e antigens. Samples phenotyped as D- were genotyped by polymerase chain reaction-sequence specific primers, and exon 10 and intron 4 of the RHD gene were analysed. D- samples containing the RHD gene or samples considered weak D were further characterised using genotyping platform or nucleotide sequencing. Using serologic methods we found that 87.3% of the donors were D+, 11.9% D- and 0.8% weak D. The frequency of RHD gene in D- individuals was 9.2%. Five RHD alleles from phenotypically D- donors were characterised in six molecular backgrounds: RHD, RHD-CE-Ds, RHD-CE-(2-9)-D, RHD/RHD, RHD/RHD-CE-Ds and RHD-CE(2)-D. The most common weak D antigens types found were 1, 3, 4.0/4.1 and 4.2, whereas the most prevalent weak D type was 4.2 (or DAR). The RHD genotyping proved to be a necessary tool to characterise RHD alleles in donors phenotyped as D- or weak D to increase the transfusion safety in highly racial mixed population. (AU)

FAPESP's process: 05/55237-9 - Clinical and molecular aspects of antigens and antibodies of blood cells
Grantee:Jose Orlando Bordin
Support Opportunities: Research Projects - Thematic Grants