Polymorphisms of the CYP1A1 and CYP2E1 genes in head and neck squamous cell carcinoma risk

Author(s)

Cury, Nathalia Moreno ^[1] ; Russo, Anelise ^[1] ; Silva Galbiatti, Ana Livia ^[1] ; Ruiz, Mariangela Torreglosa ^[1] ; Raposo, Luiz Sergio ^[2] ; Maniglia, Jose Victor ^[2] ; Pavarino, Erika Cristina ^[1] ; Goloni-Bertollo, Eny Maria ^{[1, 3]}

Affiliation

^[1] Sao Jose Do Rio Preto Med Sch FAMERP, Genet & Mol Biol Res Unit UPGEM, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Jose do Rio Preto Med Sch FAMERP, Dept Otorhinolaryngol & Head & Neck Surg, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Fac Med Sao Jose Do Rio Preto FAMERP, Dept Biol Mol, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: MOLECULAR BIOLOGY REPORTS; v. 39, n. 2, p. 1055-1063, FEB 2012.
• Web of Science Citations: 17

Abstract

Polymorphisms in genes that encode P450 cytochrome enzymes may increase carcinogen activation or decrease their inactivation and consequently, promote the development of cancer. The aims of this study were to identify the MspI-CYP1A1, PstI-CYP2E1 and DraI-CYP2E1 polymorphisms in patients with head and neck cancer and to compare with individuals without cancer; to evaluate the association of these polymorphisms with risk factors and clinical histopathological parameters. In the study group, 313 patients were evaluated for CYP1A1, 217 for CYP2E1 (PstI) and 211 for CYP2E1 (DraI) and in the control group 417, 334 and 374 individuals, respectively. Molecular analysis was performed by PCR-RFLP technique, and chi-square and multiple logistic regression tests were used for statistical analysis. The result of analysis regarding individuals evaluated for CYP1A1 (MspI) showed that age (OR: 8.15; 95% CI 5.57-11.92) and smoking (OR: 5.37; 95% CI 3.52-8.21) were predictors for the disease; for the CYP2E1 (PstI and DraI), there were associations with age (PstI-OR: 9.10; 95% CI 5.86-14.14/DraI-OR: 8.07; 95% CI 5.12-12.72), smoking (PstI-OR: 4.10; 95% CI 2.44-6.89/DraI-OR: 5.73; 95% CI 3.34-9.82), alcohol (PstI-OR: 1.93; 95% CI 1.18-3.16/DraI-OR: 1.69; 95% CI 1.02-2.81), respectively, with disease development. CYP2E1 (PstI) was less frequent in patient group (OR: 0.48; 95% CI 0.23-0.98). Regarding clinical histopathological parameters, CYP1A1 polymorphism was less frequent in the larynx primary anatomic site (OR = 0.45; 95% CI = 0.28-0.73; P = 0.014). In conclusion, we confirm that age, smoking and alcohol consumption are risk factors for this disease and the polymorphisms investigated have no association with the development of head and neck cancer. (AU)

• FAPESP's process: 07/08155-2 - Gene polymorphisms of xenobiotic-metabolizing in patients with head and neck cancer.
• Grantee: Eny Maria Goloni Bertollo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/05875-0 - Polymorphism of GSTP1 gene in head and neck cancer patients
• Grantee: Marianna Angelo Palmejani
• Support Opportunities: Scholarships in Brazil - Scientific Initiation