Preliminary Studies for Cephamycin C Purification Technique

Author(s)

de Baptista Neto, Alvaro ^[1] ; Condori Bustamante, Maritza Catalina ^[2] ; Hortolan Luiz de Oliveira, Jaine Honorata ^[3] ; Granato, Ana Claudia ^[4] ; Bellao, Carolina ^[2] ; Badino Junior, Alberto Colli ^[2] ; Barboza, Marlei ^[4] ; Hokka, Carlos Osamu ^[2]

Affiliation

^[1] Verdartis Desenvolvimento Biotecnol Ltda ME, BR-14040900 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Alfenas, Dept Pharm, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Fed Triangulo Mineiro, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 166, n. 1, p. 208-221, JAN 2012.
• Web of Science Citations: 2

Abstract

A study was made for purification of cephamycin C from fermentation of Streptomyces clavuligerus. Initially, the culture broth was clarified by microfiltration and ultrafiltration, after which the resulting permeates were subjected to nonspecific adsorption and ion-exchange chromatography on resin columns. The antibiotic activity was measured by the biological method at each stage by assaying its activity against the Escherichia coli ESS, super sensitive to beta-lactam antibiotic. The purification processes were assessed in relation to the variables affecting each step. The purification efficiency by nonspecific adsorption was monitored by UV spectrophotometry, while the ion-exchange adsorption fractions were assessed by NMR spectroscopy. Some of the fractions obtained during purification were also analyzed by mass spectrometry (LC/MS and LC/MS/MS) to identify the cephamycin C molecule. These preliminary results proved the process feasibility. (AU)

• FAPESP's process: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces
• Grantee: Carlos Osamu Hokka
• Support Opportunities: Research Projects - Thematic Grants