Application of an active attachment model as a high-throughput demineralization biofilm model

Author(s)

Silva, Thiago C. ^{[1, 2, 3, 4]} ; Pereira, Agnes Fatima F. ^{[5, 1, 2, 3]} ; Exterkate, Rob A. M. ^{[1, 3]} ; Bagnato, Vanderlei S. ^[6] ; Buzalaf, Marilia A. R. ^[5] ; Machado, Maria Aparecida de A. M. ^[4] ; ten Cate, Jacob M. ^{[1, 3]} ; Crielaard, Wim ^{[1, 3]} ; Deng, Dong Mei ^{[1, 3]}

Affiliation

^[1] Vrije Univ Amsterdam, Amsterdam - Netherlands

^[2] Univ Cuiaba, Cuiaba Sch Dent, Dept Res, BR-78065480 Cuiaba, MT - Brazil

^[3] Univ Amsterdam, Dept Prevent Dent, Acad Ctr Dent Amsterdam ACTA, Amsterdam - Netherlands

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru - Brazil

^[5] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru - Brazil

^[6] Univ Sao Paulo, Inst Phys Sao Carlos, Dept Phys & Sci Mat, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 40, n. 1, p. 41-47, JAN 2012.
• Web of Science Citations: 16

Abstract

Objectives: To investigate the potential of an active attachment biofilm model as a highthroughput demineralization biofilm model for the evaluation of caries-preventive agents. Methods: Streptococcus mutans UA159 biofilms were grown on bovine dentine discs in a highthroughput active attachment model. Biofilms were first formed in a medium with high buffer capacity for 24 h and then subjected to various photodynamic therapies (PACT) using the combination of Light Emitting Diodes (LEDs, Biotable (R)) and Photogem (R). Viability of the biofilms was evaluated by plate counts. To investigate treatment effects on dentine lesion formation, the treated biofilms were grown in a medium with low buffer capacity for an additional 24 h. Integrated mineral loss (IML) and lesion depth (LD) were assessed by transversal microradiography. Calcium release in the biofilm medium was measured by atomic absorption spectroscopy. Results: Compared to the water treated control group, significant reduction in viability of S. mutans biofilms was observed when the combination of LEDs and Photogem (R) was applied. LEDs or Photogem (R) only did not result in biofilm viability changes. Similar outcomes were also found for dentine lesion formation. Significant lower IML and LD values were only found in the group subjected to the combined treatment of LEDs and Photogem (R). There was a good correlation between the calcium release data and the IML or LD values. Conclusions: The high-throughput active attachment biofilm model is applicable for evaluating novel caries-preventive agents on both biofilm and demineralization inhibition. PACT had a killing effect on 24 h S. mutans biofilms and could inhibit the demineralization process. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 08/06969-5 - EFFECTS OF PHOTODYNAMIC THERAPY ON STREPTOCOCCUS MUTANS
• Grantee: Thiago Cruvinel da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate