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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of Cryptosporidium parvum oocysts in calf fecal samples by direct immunofluorescence assay

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Author(s):
Pires Teixeira, Weslen Fabricio [1] ; Dourado Coelho, Willian Marinho [2] ; Nunes, Caris Maroni [1] ; Meireles, Marcelo Vasconcelos [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista, Fac Med Vet, BR-16050680 Aracatuba, SP - Brazil
[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet, BR-16050680 Aracatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 20, n. 4, p. 269-273, OCT-DEC 2011.
Web of Science Citations: 3
Abstract

The aim of this study was to produce a conjugate containing anti-Cryptosporidium parvum polyclonal antibodies and standardize a Direct Immunofluorescence Assay (DIE) for detecting C parvum oocysts in fecal samples from calves. In order to obtain anti-C parvum polyclonal antibodies, two New Zealand rabbits were immunized with a purified solution of C. parvum oocysts and Freund's adjuvant. Purification of the immunoglobulin G (IgG) fraction was performed by means of precipitation in ammonium sulfate and chromatography using a DEAE-cellulose column. The anti-C parvum polyclonal antibody titer was determined by means of the enzyme-linked immunosorbent assay (ELISA). The rabbit anti-C. parvum IgG fraction was conjugated with fluorescein isothiocyanate and standardization of the DIF was performed using various dilutions of conjugate on slides positive for C. parvum oocysts. The cross-reactivity of the anti-C. parvum conjugate was tested using oocysts of Cryptosporidium serpentis. Cryptosporidium andersoni, Escherichia Eimeria sp., and Candida sp. An anti-C. parvum conjugate was successfully produced, thus allowing standardization of DIF for detection of Cryptosporidium oocysts in fecal samples. Cross-reactivity of anti-C parvum polyclonal antibodies with C. andersoni and C. serpentis was also observed. (AU)