Hydroxyl scavenging activity accounts for differential antioxidant protection of Plantago major against oxidative toxicity in isolated rat liver mitochondria

Author(s)

Mello, Joyce C. ^{[1, 2]} ; Guimaraes, Natalia S. S. ^[2] ; Gonzalez, Mariano V. D. ^[2] ; Paiva, Juliana S. ^[2] ; Prieto, Tatiana ^[3] ; Nascimento, Otaciro R. ^{[3, 1]} ; Rodrigues, Tiago ^{[1, 2]}

Affiliation

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil

^[2] Univ Mogi das Cruzes UMC, Ctr Interdisciplinar Invest Bioquim, Mogi Das Cruzes, SP - Brazil

^[3] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Journal of Pharmacy and Pharmacology; v. 64, n. 8, p. 1177-1187, AUG 2012.
• Web of Science Citations: 6

Abstract

Objectives The aim of this work was to study the effects of P. major against the oxidative damage of isolated rat liver mitochondria. Methods The extracts were obtained using methanol (MeOH), ethyl acetate (EAc), dichloromethane (DCM), and hexane (Hex) as solvents. Key findings Hex, DCM, and EAc totally, and MeOH partially, inhibited ROS generation and lipid peroxidation of membranes induced by Fe2+ or t-BOOH. However, only MeOH was able to prevent the t-BOOH-induced glutathione and NAD(P)H oxidation. All extracts chelated Fe2+ and reduced DPP Hradicals. EPR analysis revealed that P. major exhibited potent scavenger activity for hydroxyl radicals. Conclusions The potent antioxidant activity exhibited by P. major was able to prevent oxidative mitochondrial damage, contributing to the understanding of its hepatoprotective action against ROS-mediated toxicity. (AU)

• FAPESP's process: 08/01724-4 - Characterization of the Antioxidant Properties of Plant Extracts against the Oxidative Stress in Isolated Mitochondria and Cultured Cells
• Grantee: Tiago Rodrigues
• Support Opportunities: Regular Research Grants