High-yield secretion of multiple client proteins in Aspergillus

Author(s)

Segato, Fernando ^[1] ; Damasio, Andre R. L. ^[1] ; Goncalves, Thiago A. ^[2] ; de Lucas, Rosymar C. ^[1] ; Squina, Fabio M. ^[3] ; Decker, Stephen R. ^[4] ; Prade, Rolf A. ^[1]

Affiliation

^[1] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA

^[2] Univ Fed Vicosa, Dept Bioquim & Biol Mol, Vicosa, MG - Brazil

^[3] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Campinas, SP - Brazil

^[4] Natl Renewable Energy Lab, Golden, CO - USA

• Document type: Journal article
• Source: Enzyme and Microbial Technology; v. 51, n. 2, p. 100-106, JUL 15 2012.
• Web of Science Citations: 37

Abstract

Production of pure and high-yield client proteins is an important technology that addresses the need for industrial applications of enzymes as well as scientific experiments in protein chemistry and crystallization. Fungi are utilized in industrial protein production because of their ability to secrete large quantities of proteins. In this study, we engineered a high-expression-secretion vector, pEXPYR that directs proteins towards the extracellular medium in two Aspergillii host strains, examine the effect of maltose-induced over-expression and protein secretion as well as time and pH-dependent protein stability in the medium. We describe five client proteins representing a core set of hemicellulose degrading enzymes that accumulated up to 50-100 mg/L of protein. Using a recyclable genetic marker that allows serial insertion of multiple genes, simultaneous hyper-secretion of three client proteins in a single host strain was accomplished. (c) 2012 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 10/18198-3 - Systems biology of sugarcane bagasse microbial decomposition interactions
• Grantee: Fábio Márcio Squina
• Support type: Research Grants - Visiting Researcher Grant - International