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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronic periodontitis: preliminary results

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Author(s):
Miani, P. K. [1] ; do Nascimento, C. [1] ; Sato, S. [1] ; Filho, A. V. [1] ; da Fonseca, M. J. V. [2] ; Pedrazzi, V. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Dept Dent Mat & Prosthodont, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pharmaceut Sci, Sch Pharmaceut Sci, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES; v. 31, n. 7, p. 1611-1618, JUL 2012.
Web of Science Citations: 10
Abstract

The present study developed an experimental metronidazole-based gel and evaluated its efficacy for the adjuvant treatment of chronic periodontitis. Sixteen patients were randomly allocated into two groups of eight subjects according to the following proposed treatments: (1) scaling and root planing (active control) or (2) scaling and root planing and direct periodontal intrapocket application of 15% metronidazole-based gel in two sites (a parts per thousand yen5 mm in depth) (experimental group). Potential changes in the subgingival microbiota were assessed using a DNA Checkerboard method at three proposed times: baseline and following 7 or 30 days of drug administration. High-performance liquid chromatography (HPLC) monitored metronidazole concentrations in the crevicular fluid during treatment. The metronidazole experimental group presented lower bacterial counts than the control group at the three evaluated times (p < 0.01 for baseline, p < 0.001 for 7 or 30 days) when the target species were analyzed as a pool of bacteria. Samples revealed significantly lower counts 7 days after drug administration compared with baseline or after 30 days (p < 0.05). HPLC analysis detected gel 1 h after application. The metronidazole-based gel significantly decreased the total bacterial count at the three evaluated times. Periodontopathogenic species were not different after gel administration. (AU)