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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Altered immunophenotypic features of peripheral blood platelets in myelodysplastic syndromes

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Author(s):
Sandes, Alex F. [1] ; Yamamoto, Mihoko [1] ; Matarraz, Sergio [2, 3, 4] ; Chauffaille, Maria de Lourdes L. F. [1] ; Quijano, Sandra [2, 3, 4] ; Lopez, Antonio [2, 3, 4] ; Oguro, Tsutomu [5] ; Kimura, Eliza Y. S. [1] ; Orfao, Alberto [2, 3, 4]
Total Authors: 9
Affiliation:
[1] Univ Fed Sao Paulo, UNIFESP, Disciplina Hematol & Hemoterapia, Dept Oncol Clin & Expt, BR-04023900 Sao Paulo - Brazil
[2] Univ Salamanca, Dept Med, Serv Gen Citometria, E-37008 Salamanca - Spain
[3] Univ Salamanca, Canc Res Ctr IBMCC USAL CSIC, E-37008 Salamanca - Spain
[4] Univ Salamanca, IBSAL, E-37008 Salamanca - Spain
[5] Hosp Serv Publ Estadual Sao Paulo, Serv Hematol, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Haematologica; v. 97, n. 6, p. 895-902, JUN 2012.
Web of Science Citations: 19
Abstract

Background Multiparameter flow cytometric analysis of bone marrow and peripheral blood cells has proven to be of help in the diagnostic workup of myelodysplastic syndromes. However, the usefulness of flow cytometry for the detection of megakaryocytic and platelet dysplasia has not yet been investigated. The aim of this pilot study was to evaluate by flow cytometry the diagnostic and prognostic value of platelet dysplasia in myelodysplastic syndromes. Design and Methods We investigated the pattern of expression of distinct surface glycoproteins on peripheral blood platelets from a series of 44 myelodysplastic syndrome patients, 20 healthy subjects and 19 patients with platelet alterations associated to disease conditions other than myelodysplastic syndromes. Quantitative expression of CD31, CD34, CD36, CD41a, CD41b, CD42a, CD42b and CD61 glycoproteins together with the PAC-1, CD62-P, fibrinogen and CD63 platelet activation-associated markers and platelet light scatter properties were systematically evaluated. Results Overall, flow cytometry identified multiple immunophenotypic abnormalities on platelets of myelodysplastic syndrome patients, including altered light scatter characteristics, over-and under expression of specific platelet glycoproteins and asynchronous expression of CD34; decreased expression of CD36 (n=5), CD42a (n=1) and CD61 (n=2), together with reactivity for CD34 (n=1) were only observed among myelodysplastic syndrome cases, while other alterations were also found in other platelet disorders. Based on the overall platelet alterations detected for each patient, an immunophenotypic score was built which identified a subgroup of myelodysplastic syndrome patients with a high rate of moderate to severe alterations (score>1.5; n=16) who more frequently showed thrombocytopenia, megakaryocytic dysplasia and high-risk disease, together with a shorter overall survival. Conclusions Our results show the presence of altered phenotypes by flow cytometry on platelets from around half of the myelodysplastic syndrome patients studied. If confirmed in larger series of patients, these findings may help refine the diagnostic and prognostic assessment of this group of disorders. (AU)