ANTI-MYCOBACTERIUM TUBERCULOSIS ACTIVITY OF FUNGUS PHOMOPSIS STIPATA

Author(s)

de Prince, Karina Andrade ^{[1, 2]} ; Sordi, Renata ^[3] ; Pavan, Fernando Rogerio ^[1] ; Barreto Santos, Adolfo Carlos ^[1] ; Araujo, Angela R. ^[3] ; Leite, Sergio R. A. ^[3] ; Leite, Clarice Q. F. ^[1]

Affiliation

^[1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Araraquara, SP - Brazil

^[2] Fac Integradas Pitagoras, Montes Claros, MG - Brazil

^[3] Univ Estadual Paulista, Inst Quim, Araraquara, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 43, n. 1, p. 224-229, JAN-MAR 2012.
• Web of Science Citations: 3

Abstract

Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties. (AU)

• FAPESP's process: 11/11593-7 - Research for new drugs against tuberculosis: laboratory improvement to perform pre-clinical trials
• Grantee: Fernando Rogério Pavan
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 08/10390-2 - Determination of pre-clinical profile in vitro and in vivo anti-tuberculosis activity of ruthenium(II)phosphine / picolinate complexes
• Grantee: Fernando Rogério Pavan
• Support Opportunities: Scholarships in Brazil - Doctorate