Doxycycline ameliorates the dystrophic phenotype of skeletal and cardiac muscles in mdx mice

Author(s)

Pereira, Juliano Alves ^[1] ; Tiemi Taniguti, Ana Paula ^[1] ; Matsumura, Cintia ^[1] ; Marques, Maria Julia ^[1] ; Neto, Humberto Santo ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Anat Biol Celular Fisiol & Biofis, UNICAMP, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: MUSCLE & NERVE; v. 46, n. 3, p. 400-406, SEP 2012.
• Web of Science Citations: 8

Abstract

Introduction: We examined whether doxycycline, an antibiotic member of the tetracycline family, improves the histopathology and muscle function in mdx mice, the experimental model of DMD. Methods: Doxycycline was administered for 36 days (starting on postnatal day 0) and for 9 months (starting at 8 months of age) in drinking water. Histopathological, biochemical (creatine kinase), and functional (forelimb muscle grip strength) parameters were evaluated in limb, diaphragm, and cardiac muscle. Results: Doxycycline significantly minimized the dystrophic phenotype of skeletal and cardiac muscles and improved forelimb muscle strength. The drug protected muscle fibers against myonecrosis and reduced inflammation. Furthermore, it slowed the progression of myocardial fibrosis. Conclusions: This study provides evidence that doxycycline may be a potential therapeutic agent for DMD. Muscle Nerve 46: 400406, 2012 (AU)