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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The pattern recognition receptors expressed on neutrophils and the associated cytokine profile from different aged patients with Candida-related denture stomatitis

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Author(s):
Gasparoto, Thais Helena [1] ; de Oliveira, Carine Ervolino [2] ; Vieira, Narciso Almeida [3] ; Porto, Vinicius Carvalho [4] ; Gasparoto, Carlos Teodoro [5] ; Campanelli, Ana Paula [1] ; Lara, Vanessa Soares [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Dept Stomatol Oral Pathol, Bauru Sch Dent, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Clin Anal Lab, Hosp Rehabil Craniofacial Anomalies, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Sch Publ Hlth, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Experimental Gerontology; v. 47, n. 9, p. 741-748, SEP 2012.
Web of Science Citations: 8
Abstract

This study evaluated the expression of pattern recognition receptors (PRRs) and activation factors associated with salivary and blood neutrophils from different aged patients diagnosed with Candida-related denture stomatitis (DS). Expression of neutrophil PRRs was determined by flow cytometry and immunofluorescence, and the levels of selected cytokines that influence immune activation were determined by ELISA. The salivary (but not the serum derived) neutrophils of individuals with DS were found to have an increased expression of CD69 regardless of the age of the patient compared to patients without DS. However, these salivary neutrophils had a lower expression of CD66b and CD64. Expression of TLR2 was lower on the salivary-and serum-derived neutrophils from elderly individuals compared to the neutrophils of younger subjects, regardless of whether the individual had DS. Salivary interleukin (IL)-4 was elevated in both of the elderly subject groups (with or without DS). Only elderly DS patients were observed to have increased serum IL-4 levels and reduced salivary IL-12 levels. Younger DS patients showed an increase in salivary IL-10 levels, and both the saliva and the serum levels of IFN-gamma were increased in all of the younger subjects. Our data demonstrated that changes in both the oral immune cells and the protein components could be associated with DS. Furthermore, changes in the blood-derived factors were more associated with age than DS status. (C) 2012 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 05/60668-9 - Study of neutrophils from elderly in the defense mechanisms against Candida-related denture stomatitis
Grantee:Thaís Helena Gasparoto
Support Opportunities: Scholarships in Brazil - Doctorate