Influence of Fixation Products Used in the Histological Processing in the FTIR Spectra of Lung Cells

Author(s)

Pereira, Thiago Martini ^[1] ; Zaidan Dagli, Maria Lucia ^[2] ; Mennecier, Gregory ^[2] ; Zezell, Denise Maria ^[1]

Affiliation

^[1] Inst Pesquisas Energeticas & Nucl CNEN SP, Ctr Lasers & Aplicacoes, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Patol, Fac Med Vet & Zootecnia, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: SPECTROSCOPY-AN INTERNATIONAL JOURNAL; v. 27, n. 5-6, p. 399-402, 2012.
• Web of Science Citations: 4

Abstract

The aim of the present study is to evaluate the differences on FTIR spectra of the normal lung cell (noncancerous mice lung epithelial cell line e10) due to different fixation protocols for histological processing. The results shown that formalin and methacarn (normally used in fixation) did cause many changes on the FTIR spectra of mice lung cells e10, mainly in the organic compounds (800-1800 cm(-1)) in lipids, DNA, and proteins, and the alcohol 70% fixation protocol caused almost no changes on the FTIR spectra compared to unfixed cells spectra (in PBS). It can be concluded that histological processing with alcohol 70% fixation protocol can be used in the FTIR study of mice lung cell line e10. (AU)

• FAPESP's process: 05/51689-2 - Optics and Photonics Research Center at UNICAMP
• Grantee: Hugo Luis Fragnito
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC