Effect of low-level laser therapy on intramembranous and endochondral autogenous bone grafts healing

Author(s)

Biguetti, Claudia Cristina ^[1] ; Marquardt Filho, Emilio Jose ^[2] ; Holgado, Leandro De Andrade ^[3] ; Caviquioli, Gustavo ^[1] ; Moreschi, Eduardo ^[4] ; Comparin, Eliston ; Matsumoto, Mariza Akemi ^[5]

Affiliation

^[1] Sagrado Coracao Univ USC, Sch Dent, BR-17011160 Sao Paulo - Brazil

^[2] Sagrado Coracao Univ USC, Oral & Maxillofacial Surg Masters Course Program, BR-17011160 Sao Paulo - Brazil

^[3] Sagrado Coracao Univ USC, Dept Hlth Sci, BR-17011160 Sao Paulo - Brazil

^[4] Sagrado Coracao Univ USC, Oral & Maxillofacial Surg Doctorals Course Progra, Ctr Univ Maringa CESUMAR, BR-17011160 Sao Paulo - Brazil

^[5] Sagrado Coracao Univ USC, Dept Hlth Sci, BR-17013070 Sao Paulo - Brazil

• Document type: Journal article
• Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 75, n. 9, p. 1237-1244, SEP 2012.
• Web of Science Citations: 3

Abstract

The aim of this study was to evaluate the healing process of intramembranous (IM) and endochondral (EC) bone grafts under low-level laser therapy (LLLT). Male rabbits underwent onlay autogenous bone grafts (1 cm in diameter) retrieved from the calvaria and iliac crest and fixed on parietal bones, divided into four groups: Calvaria (C), Iliac (I), Calvaria + LLLT (C+L), and Iliac + LLLT (I+L). Animals from C+L and I+L Groups had their grafts exposed to LLLT (AlGaAs808 nm, CW, 30 mW, 0.028 cm2 average laser beam area), 15 s irradiation time (16 J/cm2 per pointtotal of 64 J/cm2 per session). After 7, 14, 30, and 60 days, grafts were retrieved and resorption pattern analyzed by means of morphometry and TRAP-positive osteoclasts detection. Differences in the resorption levels of iliac grafts were observed, presenting 40% in I group against 8% in I+L grafts at the 14th day of evaluation (P < 0.05). After 30 days, resorption was maintained at 41% in I group, whereas I+L presented 15% in the same period (P = 0.0591). No significant differences were noted in the rates of calvaria grafts resorption in all periods. A significant higher number of osteoclasts on the grafts' surface was observed in C+L Group at day 30, in comparison with C group. In I+L Group, prevalence of osteoclasts was marked at day 7 (P < 0.05) in comparison to I Group. In general, it was concluded that biomodulative effects of LLLT did not significantly affect healing and resorption processes of autogenous bone grafts from EC and IM origins. Microsc. Res. Tech. 75:12371244, 2012. (C) 2012 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 09/14989-9 - Influence of cyclooxygenase 2 enzyme on autogenous bone grafts repair: histomorphometric analysis in rabbits
• Grantee: Claudia Cristina Biguetti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 08/11485-7 - Immunohistochemical analysis of autogenous bone grafts repair under low-level lasertherapy: study in rabbits
• Grantee: Mariza Akemi Matsumoto
• Support Opportunities: Regular Research Grants