Lymphocyte proliferation stimulated by activated Cebus apella macrophages treated with a complex homeopathic immune response modifiers

Author(s)

Coelho Moreira, Carlos Onete ^{[1, 2]} ; Ferreira Borges da Costa, Joana de Fatima ^[1] ; Leal, Mariana Ferreira ^[3] ; de Andrade, Edilson Ferreira ^[1] ; Rezende, Alexandre Pingarilho ^[1] ; Imbeloni, Aline Amaral ^[4] ; Pereira Carneiro Muniz, Jose Augusto ^[4] ; Cardoso Smith, Marilia de Arruda ^[3] ; Burbano, Rommel Rodriguez ^[1] ; de Assumpcao, Paulo Pimentel ^[2]

Affiliation

^[1] Fed Univ Para, Lab Citogenetica Humana, Inst Ciencias Biol, BR-66059 Belem, PA - Brazil

^[2] Fed Univ Para, Serv Cirurgia, Hosp Univ Joao de Barros Barreto, BR-66073000 Belem, PA - Brazil

^[3] Univ Fed Sao Paulo, Discipline Genetica, Dept Morfol & Genetica, BR-04023900 Sao Paulo - Brazil

^[4] Minist Saude, Ctr Nacl Primatas, BR-77030000 Ananindeua, PA - Brazil

• Document type: Journal article
• Source: Homeopathy; v. 101, n. 1, p. 74-79, JAN 2012.
• Web of Science Citations: 8

Abstract

Introduction: Canova is a complex homeopathic medicine that enhances a specific immunologic responses against several exogenous and endogenous conditions. Canova activates macrophages both in vivo and in vitro. Aim and method: We evaluated the effects of macrophages activated by Canova in vivo and ex vitro in the proliferation of lymphocytes. Canova was used to activate Cebus apella macrophages in vivo or ex vitro with Canova. Lymphocytes were cultured with the macrophage culture medium. The analysis of Canova effects in cultured lymphocytes was performed according to the cell cycle phase using flow cytometry. The Interferon gamma and Interleukin-5 cytokines quantification in these lymphocyte culture media was performed by Enzyme-linked immunosorbent assay (ELISA). Results: We observed that Canova actives macrophages in vivo and ex vitro. The lymphocytes cultured in a supplemented medium with macrophages activated by Canova treatment presented a higher number of proliferation cells than lymphocytes not exposed to macrophages activated by Canova. The Interferon gamma and Interleukin-5 cytokines were only observed in the medium of lymphocytes exposed to macrophages activated by Canova. Thus, Canova has potential as a new adjuvant therapy. Homeopathy (201 2) 101, 74-79. (AU)

• FAPESP's process: 07/02470-3 - Differential phospho and glyco proteomic analysis in gastric adenocarcinoma and the eventual correlation with clinic-pathological parameters of prognosis
• Grantee: Mariana Ferreira Leal
• Support type: Scholarships in Brazil - Doctorate