Pivotal Role of Toll-Like Receptors 2 and 4, Its Adaptor Molecule MyD88, and Inflammasome Complex in Experimental Tubule-Interstitial Nephritis

Author(s):Show less -

Correa-Costa, Matheus ^[1] ; Braga, Tarcio Teodoro ^[1] ; Semedo, Patricia ^[2] ; Hayashida, Caroline Yuri ^[1] ; Grassmann Bechara, Luiz Roberto ^[3] ; Elias, Rosa Maria ^[2] ; Barreto, Claudiene Rodrigues ^[2] ; Silva-Cunha, Claudia ^[1] ; Hyane, Meire Ioshie ^[1] ; Goncalves, Giselle Martins ^[1] ; Brum, Patricia Chakur ^[3] ; Fujihara, Clarice ^[4] ; Zatz, Roberto ^[4] ; Pacheco-Silva, Alvaro ^{[2, 5]} ; Zamboni, Dario S. ^[6] ; Saraiva Camara, Niels Olsen ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci 4, Dept Immunol, Lab Transplantat Immunobiol, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Div Nephrol, Lab Clin & Expt Immunol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, Dept Clin Med, Div Renal, Sao Paulo - Brazil

^[5] Albert Einstein Hosp, Renal Transplantat Unit, Inst Israelita Ensino & Pesquisa Albert Einstein, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Cell Biol, Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS One; v. 6, n. 12 DEC 14 2011.
• Web of Science Citations: 47

Abstract

Tubule-interstitial nephritis (TIN) results in decreased renal function and interstitial inflammation, which ultimately leads to fibrosis. Excessive adenine intake can cause TIN because xanthine dehydrogenase (XDH) can convert this purine into an insoluble compound, which precipitates in the tubuli. Innate immune sensors, such as Toll-like receptors (TLR) and inflammasome complex, play a crucial role in the initiation of inflammation. The aim of this study was to evaluate the roles of TLR-2 and -4, Myd88 and inflammasome complex in an experimental model of TIN. Here, we show that wild-type (WT) mice fed adenine-enriched food exhibited significant renal dysfunction and enhanced cellular infiltration accompanied by collagen deposition. They also presented higher gene and protein expression of pro-inflammatory cytokines. In contrast, TLR-2, -4, MyD88, ASC and Caspase-1 KO mice showed renoprotection associated with expression of inflammatory molecules at levels comparable to controls. Furthermore, treatment of WT animals with allopurinol, an XDH inhibitor, led to reduced levels of uric acid, oxidative stress, collagen deposition and a downregulation of the NF-kB signaling pathway. We concluded that MyD88 signaling and inflammasome participate in the development of TIN. Furthermore, inhibition of XDH seems to be a promising way to therapeutically target the developing inflammatory process. (AU)

• FAPESP's process: 07/07139-3 - The role of heme oxygenase 1 in different renal inflammatory process in experimental animal models
• Grantee: Niels Olsen Saraiva Câmara
• Support Opportunities: Research Projects - Thematic Grants