Expression Profiling of Cell Cycle Regulatory Proteins in Oropharyngeal Carcinomas Using Tissue Microarrays

Author(s)

Ribeiro, Daniel A. ^{[1, 2]} ; Nascimento, Fabio D. ^[2] ; Fracalossi, Ana Carolina C. ^[2] ; Gomes, Thiago S. ^[2] ; Oshima, Celina T. F. ^[2] ; Franco, Marcello F. ^[2]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biociencias, BR-11060001 Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Patol, BR-11060001 Santos, SP - Brazil

• Document type: Journal article
• Source: IN VIVO; v. 24, n. 3, p. 315-320, MAY-JUN 2010.
• Web of Science Citations: 4

Abstract

Aim: The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis. Patients and Methods: Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays. Results: Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx. Conclusion: Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry. (AU)

• FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
• Grantee: Daniel Araki Ribeiro
• Support Opportunities: Research Grants - Young Investigators Grants