From protein interaction profile to functional assignment The human protein Ki-1/57 is associated with pre-mRNA splicing events

Author(s)

Bressan, Gustavo Costa ^[1] ; Kobarg, Joerg ^[1]

Affiliation

^[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias, Sao Paulo - Brazil

• Document type: Journal article
• Source: RNA BIOLOGY; v. 7, n. 3, p. 268-271, MAY-JUN 2010.
• Web of Science Citations: 3

Abstract

The mapping of protein-protein interactions of a determined organism is considered fundamental to assign protein function in the post-genomic era. As part of this effort, screenings for pairwise interactions by yeast two-hybrid system have been used popularly to reveal protein interaction networks in different biological systems. Through the identification of protein interaction partners we have successfully obtained interesting functional clues for Ki-1/57, a human protein with no previous functional annotation, in the context of RNA metabolism. We briefly discuss here the way we approached protein-protein interaction data to conduct and interpret further molecular biological and cellular studies as well as structural analyses on this protein. Our data suggest that Ki-1/57 belongs to the family of intrinsically unstructured proteins and that the structural flexibility may be crucial for its capacity to interact with many different proteins. A large fraction of these proteins are involved in pre-mRNA splicing control. Finally, Ki-1/57 is localized to several subnuclear domains, all of which have been described to splicing and other RNA processing events. (AU)

• FAPESP's process: 08/04849-2 - Functional and structural studies of human proteins involved in cancer
• Grantee: Jörg Kobarg
• Support Opportunities: Regular Research Grants