Levels of Selenomonas species in generalized aggressive periodontitis

Author(s)

Goncalves, L. F. H. ; Fermiano, D. ; Feres, M. ; Figueiredo, L. C. ; Teles, F. R. P. ^[1] ; Mayer, M. P. A. ^[2] ; Faveri, M. ^{[2, 3]}

Affiliation

^[1] Forsyth Inst, Dept Periodontol, Cambridge, MA - USA

^[2] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, Sao Paulo - Brazil

^[3] Univ Guarulhos, Ctr Posgrad & Pesquisa CEPPE, Dept Periodontol, Dent Res Div, BR-07023070 Guarulhos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PERIODONTAL RESEARCH; v. 47, n. 6, p. 711-718, DEC 2012.
• Web of Science Citations: 25

Abstract

Goncalves LFH, Fermiano D, Feres M, Figueiredo LC, Teles FRP, Mayer MPA, Faveri M. Levels of Selenomonas species in generalized aggressive periodontitis. J Periodont Res 2012; 47: 711718. (c) 2012 John Wiley \& Sons A/S Background and Objective: To compare the levels of Selenomonas sputigena and uncultivated/unrecognized Selenomonas species in subgingival biofilms from periodontally healthy subjects and from subjects with generalized aggressive periodontitis. Material and Methods: Fifteen periodontally healthy subjects and 15 subjects with generalized aggressive periodontitis were recruited and their clinical periodontal parameters were evaluated. Nine subgingival plaque samples were collected from each subject and all were individually analyzed for the levels of 10 bacterial taxa, including cultured and uncultivated/unrecognized microorganisms, using the RNA-oligonucleotide quantification technique. Between-group differences in the levels of the test taxa were determined using the MannWhitney U-test. Results: Subjects with generalized aggressive periodontitis showed significantly higher mean counts of Porphyromonas gingivalis, S. sputigena and the Mitsuokella sp. Human Oral Taxon (HOT) 131 (previously described as Selenomonas sp. oral clone CS002), while higher mean counts of Actinomyces gerencseriae and Streptococcus sanguinis were found in periodontally healthy subjects (p < 0.01). Selenomonas sp. HOT 146 was only detected in the generalized aggressive periodontitis group. In the generalized aggressive periodontitis group, the levels of P.gingivalis and S.sputigena were higher in deep sites (probing depth = 5 mm) than in shallow sites (probing depth = 3 mm) (p < 0.01). Furthermore, in subjects with generalized aggressive periodontitis, sites with probing depth of = 3 mm harbored higher levels of these two species than sites with the same probing depth in periodontally healthy subjects. There were positive correlations between probing depth and the levels of P.gingivalis (r = 0.77; p < 0.01), S.sputigena (r = 0.60; p < 0.01) and Selenomonas dianae (previously described as Selenomonas sp. oral clone EW076) (r = 0.42, p < 0.05). Conclusion: S. sputigena and Mitsuokella sp. HOT 131 may be associated with the pathogenesis of generalized aggressive periodontitis, and their role in the onset and progression of this infection should be investigated further. (AU)

• FAPESP's process: 09/12358-1 - Prevalence of not yet-cultivated and unusual bacterial species in subjects with chronic periodontitis by 16S rRNA Checkerboard DNA-DNA Hybridization
• Grantee: Marcelo de Faveri
• Support Opportunities: Regular Research Grants