Effect of low-level laser therapy on proliferation, differentiation, and adhesion of steroid-treated osteoblasts

Author(s)

Nogueira, Gustavo Tralli ^[1] ; Mesquita-Ferrari, Raquel Agnelli ^[2] ; Costa Souza, Nadhia Helena ^[1] ; Artilheiro, Paola Pelegrineli ^[1] ; Albertini, Regiane ^[2] ; Bussadori, Sandra Kalil ^[2] ; Santos Fernandes, Kristianne Porta ^[2]

Affiliation

^[1] Univ Nove Julho, Dept Rehabil Sci, BR-05001100 Sao Paulo - Brazil

^[2] Univ Nove Julho, Masters Course Rehabil Sci, BR-05001100 Sao Paulo - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 27, n. 6, p. 1189-1193, NOV 2012.
• Web of Science Citations: 14

Abstract

There has recently been constant effort to evaluate therapies that may have a positive effect on bone regeneration. However, there are few studies in the literature on the effects of low-level laser therapy (LLLT) involving tissues treated with anabolic steroids. The present study evaluated the effects of LLLT (AsGaAl 780 nm, 3 J/cm(2), 10 mW, beam spot of 0.04 cm(2), total energy 0.12 J) on the proliferation, adhesion, and differentiation of osteoblasts cultured in the presence of nandrolone decanoate (ND). The MTT method was employed to evaluate cell proliferation and adhesion. Cell differentiation was evaluated by measuring alkaline phosphatase activity. There was a significant decrease in cell proliferation in the irradiated group treated with 50 mu M ND when compared to the control group, after 48 h. After 72 h, cell proliferation was significantly greater in the control group than in the irradiated groups treated with the steroid at concentrations of 10, 25, and 50 mu M. With regard to cell differentiation, alkaline phosphatase activity was significantly higher in the irradiated group treated with 50 mu M ND than in the control group, irradiated non-treated group, and irradiated group treated with 25 mu M ND. After 60 min of plating, the irradiated non-treated group and irradiated groups treated with the steroid at concentrations of 5, 10, and 25 mu M exhibited a significant increase in cell adhesion compared to the control group. LLLT in combination with a high concentration of steroid inhibited cell proliferation, possibly by inducing cell differentiation, while irradiation combined with lower concentrations of the steroid induced an increase in cell adhesion. (AU)

• FAPESP's process: 10/06116-2 - Effect of anabolic steroid nandrolone decanoate on the proliferation and differentiation of osteoblasts
• Grantee: Nadhia Helena Costa Souza
• Support Opportunities: Scholarships in Brazil - Scientific Initiation