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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic polymorphisms and surface expression of CTLA-4 and PD-1 on T cells of silica-exposed workers

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Author(s):
Rocha, Michelle C. [1] ; Santos, Leonilda M. B. [2] ; Bagatin, Ericson [3] ; Tervaert, Jan W. Cohen [4] ; Damoiseaux, Jan G. M. C. [5] ; Lido, Alessandro V. [3] ; Longhini, Ana L. [2] ; Torello, Cristiane O. [1] ; Queiroz, Mary L. S. [1]
Total Authors: 9
Affiliation:
[1] State Univ Campinas UNICAMP, FCM, Fac Med Sci, Dept Pharmacol & Hemoctr, BR-13083878 Campinas, SP - Brazil
[2] State Univ Campinas UNICAMP, Inst Biol, Neuroimmunol Unit, Dept Genet, BR-13083878 Campinas, SP - Brazil
[3] State Univ Campinas UNICAMP, FCM, Fac Med Sci, Occupat Med Area, Dept Prevent & Social Med, BR-13083878 Campinas, SP - Brazil
[4] Maastricht Univ, Med Ctr, Div Clin & Expt Immunol, Dept Internal Med, Maastricht - Netherlands
[5] Maastricht Univ, Med Ctr, Clin Immunol Lab, Maastricht - Netherlands
Total Affiliations: 5
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH; v. 215, n. 6, p. 562-569, NOV 2012.
Web of Science Citations: 8
Abstract

Exposure to silica dust has been examined as a possible risk factor for autoimmune diseases, including scleroderma, rheumatoid arthritis and systemic lupus erythematosus. Since CTLA-4 {[}CD152] and PD-1 {[}CD279] are important for the maintenance of peripheral tolerance by regulating T cell responsiveness, we evaluated the expression of these molecules on the surface of CD4 and CD8 T cells, as well as single nucleotide polymorphisms (SNP) in CTLA-4 and PDCD1 genes, of 70 silica-exposed workers and 30 non-exposed, age-, ethnically- and sex-matched controls. Expression of CTLA-4 was significantly (P < 0.05) reduced in CD4 T cells of exposed individuals {[}median = 0.1% and interquartile range, IQR 0.0-0.1% (exposed), median = 0.20%, IQR 0.0-0.4% (control)]. Also the expression of PD-1 was significantly (P<0.0001) reduced in both CD4 {[}median = 0.9%, IQR 0.4-2.3% (exposed), median = 5.7%, IQR 1.4-13.3% (control)] and CD8 T cells {[}median = 0.9%, IQR 0.3-1.9% (exposed), median = 5.0%, IQR 3.4-8.9% (control)]. The study of polymorphisms demonstrated a lower frequency of the A allele in the analysis of the PD1.3 SNP in the exposed group, which might be associated with the lower expression of PD-1 on the surface of CD4 T cells. Our findings provide evidence for the association of silica exposure and the maintenance of self-tolerance, i.e., the susceptibility to autoimmune disorders. (C) 2011 Elsevier GmbH. All rights reserved. (AU)

FAPESP's process: 01/13823-8 - Immunotoxicological changes and antioxidant system in erytrocytes and neutrophils in silica-exposed workers
Grantee:Mary Luci de Souza Queiroz
Support Opportunities: Research Projects - Thematic Grants