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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver

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Author(s):
Oliveira-Filho, Jose P. [1] ; Marques, Jessica A. [1] ; Cunha, Paulo Henrique J. [2] ; Medeiros, Gildenor X. [3] ; Riet-Correa, Franklin [3] ; Machado, Vania Maria V. [1] ; Borges, Alexandre S. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista Unesp, Fac Med Vet & Zootecnia, Dept Clin Vet, BR-18618000 Botucatu, SP - Brazil
[2] Univ Fed Goias UFG, Escola Vet, Dept Med Vet, BR-74001970 Goiania, Go - Brazil
[3] Univ Fed Campina Grande UFCG, Hosp Vet, BR-58700000 Patos de Minas, PB - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 32, n. 10, p. 1050-1054, OCT 2012.
Web of Science Citations: 0
Abstract

The hypoferremia that is observed during systemic inflammatory processes is mediated by hepcidin, which is a peptide that is mainly synthesized in the livers of several mammalian species. Hepcidin plays a key role in iron metabolism and in the innate immune system. It's up-regulation is particularly useful during acute inflammation, and it restricts the iron availability that is necessary for the growth of pathogenic microorganisms. In this study, the hepcidin mRNA of Equus asinus has been characterized, and the expression of donkey hepcidin in the liver has been determined. The donkey hepcidin sequence has an open reading frame (ORF) of 261 nucleotides, and the deduced corresponding protein sequence has 86 amino acids. The amino acid sequence of donkey hepcidin was most homologous to Equus caballus (98%). The mature donkey hepcidin sequence (25 amino acids) was 100% homologous to the equine mature hepcidin and has eight conserved cysteine residues that are found in all of the investigated hepcidin sequences. The expression profile of donkey hepcidin in the liver was high and was similar to the reference gene expression. The donkey hepcidin sequence was deposited in GenBankTM (HQ902884) and may be useful for additional studies on iron metabolism and the inflammatory process in this species. (AU)

FAPESP's process: 10/00058-0 - SEQUENCING AND CHARACATERIZATION OF HEPCIDIN GENE IN DONKEY (Equus asinus).
Grantee:Jéssica de Assis Marques
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 07/07344-6 - Effects of equine inflammation on hepcidin expression and plasma iron concentration
Grantee:Alexandre Secorun Borges
Support Opportunities: Regular Research Grants