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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Production of clavulanic acid by Streptomyces clavuligerus in batch cultures without and with glycerol pulses under different temperature conditions

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Author(s):
Costa, Cecilia L. L. [1] ; Badino, Alberto C. [1]
Total Authors: 2
Affiliation:
[1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Biochemical Engineering Journal; v. 69, p. 1-7, DEC 15 2012.
Web of Science Citations: 6
Abstract

Clavulanic acid (CA) is a potent beta-lactamase inhibitor produced by Streptomyces clavuligerus. Like other beta-lactam compounds, CA is chemically unstable at high temperatures. The decomposition of CA during bacterial fermentation reduces its concentration in the broth, resulting in low yields. This study investigated the use of temperature reduction to obtain high CA production during fermentation employing cultures of S. clavuligerus. Simple batch cultures, and batch cultures with glycerol pulses, were performed at different temperatures in shake flasks at 250 rpm, and pH 6.8. Firstly, three batch cultures using glycerol as carbon source were carried out at temperatures of 30 (control), 25, and 20 degrees C. Next, three batch cultures were carried out with temperature reductions from 30 to 25 degrees C, 30 to 20 degrees C, and 25 to 20 degrees C, after glycerol exhaustion. CA production generally increased in cultures with temperature reduction and glycerol feeding. Maximum CA concentration of 1534.3 mg L-1 was achieved in a culture maintained at a constant temperature of 20 degrees C, with one pulse of glycerol. Under these conditions, both glycerol uptake rate and CA degradation were low. The results obtained demonstrate the potential of temperature reduction in the optimization of CA production by S. clavuligerus. (C) 2012 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/23807-1 - Development and evaluation of the operational conditions of bioreactors for production of biotechnological products
Grantee:Alberto Colli Badino Junior
Support Opportunities: Regular Research Grants