Low Sensitivity of NS1 Protein Tests Evidenced during a Dengue Type 2 Virus Outbreak in Santos, Brazil, in 2010

Author(s):Show less -

Felix, Alvina Clara ^{[1, 2]} ; Romano, Camila Malta ^{[1, 2]} ; Centrone, Cristiane de Campos ^{[1, 2]} ; Rodrigues, Celia Lima ^{[1, 2]} ; Villas-Boas, Lucy ^{[1, 2]} ; Araujo, Evaldo Stanislau ^{[1, 2, 3]} ; de Matos, Andreia Manso ^[4] ; Carvalho, Karina Inacio ^[4] ; Turchi Martelli, Celina Maria ^[5] ; Kallas, Esper Georges ^[4] ; Pannuti, Claudio Sergio ^{[1, 2]} ; Levi, Jose Eduardo ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas Parasitarias LIMHC, Sao Paulo - Brazil

^[3] Hosp Ana Costa, Santos - Brazil

^[4] Univ Sao Paulo, Fac Med, Disciplina Imunol Clin & Alergia LIM 60, Sao Paulo - Brazil

^[5] Univ Fed Goias, IPTSP, Goiania, Go - Brazil

• Document type: Journal article
• Source: Clinical and Vaccine Immunology; v. 19, n. 12, p. 1972-1976, DEC 2012.
• Web of Science Citations: 23

Abstract

In 2010, a large outbreak of dengue occurred in Santos, Brazil. The detection of the NS1 antigen was used for diagnosis in addition to the detection of IgG, IgM, and RNA. A large number of NS1 false-negative results were obtained. A total of 379 RNA-positive samples were selected for thorough evaluation. NS1 was reactive in 37.7% of cases. Most of the cases were characterized as a secondary infection by dengue 2 virus. Sequencing of NS1 positive and negative isolates did not reveal any mutation that could justify the diagnostic failure. Use of existing NS1 tests in the Brazilian population may present a low negative predictive value, and they should be used with caution, preferentially after performing a validation with samples freshly obtained during the ongoing epidemic. (AU)

• FAPESP's process: 10/12313-5 - Analysis of inter and intra-host genetic variability of dengue virus serotype 2 (DENV2) from patients presenting dengue fever and dengue hemorrhagic fever
• Grantee: Camila Malta Romano
• Support Opportunities: Regular Research Grants