| Full text | |
| Author(s): Show less - |
Felix, Alvina Clara
[1, 2]
;
Romano, Camila Malta
[1, 2]
;
Centrone, Cristiane de Campos
[1, 2]
;
Rodrigues, Celia Lima
[1, 2]
;
Villas-Boas, Lucy
[1, 2]
;
Araujo, Evaldo Stanislau
[1, 2, 3]
;
de Matos, Andreia Manso
[4]
;
Carvalho, Karina Inacio
[4]
;
Turchi Martelli, Celina Maria
[5]
;
Kallas, Esper Georges
[4]
;
Pannuti, Claudio Sergio
[1, 2]
;
Levi, Jose Eduardo
[1, 2]
Total Authors: 12
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas Parasitarias LIMHC, Sao Paulo - Brazil
[3] Hosp Ana Costa, Santos - Brazil
[4] Univ Sao Paulo, Fac Med, Disciplina Imunol Clin & Alergia LIM 60, Sao Paulo - Brazil
[5] Univ Fed Goias, IPTSP, Goiania, Go - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Clinical and Vaccine Immunology; v. 19, n. 12, p. 1972-1976, DEC 2012. |
| Web of Science Citations: | 23 |
| Abstract | |
In 2010, a large outbreak of dengue occurred in Santos, Brazil. The detection of the NS1 antigen was used for diagnosis in addition to the detection of IgG, IgM, and RNA. A large number of NS1 false-negative results were obtained. A total of 379 RNA-positive samples were selected for thorough evaluation. NS1 was reactive in 37.7% of cases. Most of the cases were characterized as a secondary infection by dengue 2 virus. Sequencing of NS1 positive and negative isolates did not reveal any mutation that could justify the diagnostic failure. Use of existing NS1 tests in the Brazilian population may present a low negative predictive value, and they should be used with caution, preferentially after performing a validation with samples freshly obtained during the ongoing epidemic. (AU) | |
| FAPESP's process: | 10/12313-5 - Analysis of inter and intra-host genetic variability of dengue virus serotype 2 (DENV2) from patients presenting dengue fever and dengue hemorrhagic fever |
| Grantee: | Camila Malta Romano |
| Support Opportunities: | Regular Research Grants |