Dengue (DENV), Zika (ZIKV) and Chikungunya (CHIV) are the most evident arboviruses in the current international scenario. These three viruses are transmitted by mosquitoes of the Aedes genus and there are face difficulties regarding the generation of accurate epidemiological data when they co-circulate in the same geographic region, as in Brazil. This fact is ascribed to the high similarity of clinical symptoms of the diseases and the extensive cross-reaction observed in serological tests. The availability of sensitive, specific and low cost serological tests, especially for ZIKV and CHIV, is still limited in the country, thus, the aim of the present project is to develop uniplex (ELISA, IFA) and multiplex (Luminex) serological tests based on the use of recombinant forms of structural and non-structural proteins of DENV, ZIKV and CHIV. The emergence of the zika network during the ZIKV outbreak in São Paulo resulted in a strong effort to develop serological tests with high sensitivity and specificity against DENV and ZIKV. As a consequence, recombinant forms of envelope (E) and non-structural (NS1) protein portions that have low homology between the three viruses will be produced. After the antigenic evaluation, the proteins will be used in serodiagnostic platforms that can be easily translated into products (ELISA and IFA) and implemented in the large scale diagnostic laboratories and health centers of the country. The validations of the specific serological tests generated will be carried out with human and nonhuman primates serum libraries already provided by collaborators. Thus, it is expected that the knowledge generated during this thesis will contribute to the development of different serodiagnostic methods specific for DENV, ZIKV and CHIV. (AU)
News published in Agência FAPESP Newsletter about the scholarship:
DOS SANTOS ALVES, RUBENS PRINCE;
PEREIRA, SARA ARAUJO;
PEREIRA, LENNON RAMOS;
DE FREITAS, CARLA LONGO;
PEREIRA, SAMUEL SANTOS;
VENCESLAU-CARVALHO, ALEXIA ADRIANNE;
CASTRO-AMARANTE, MARIA FERNANDA;
PINHO FAVARO, MARIANNA TEIXEIRA;
AMORIM, JAIME HENRIQUE;
DE SOUZA FERREIRA, LUIS CARLOS.
Transcutaneous Administration of Dengue Vaccines.
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