Quantitative histological analysis of the mandibular branch of the facial nerve in rats

Author(s)

Zabeu Rossi Costa, Heloisa Juliana ^{[1, 2]} ; Salomone, Raquel ^[3] ; da Silva, Ciro Ferreira ^[4] ; Costa, Marcio Paulino ^[5] ; Ramos, Beatriz Lucchetta ^[3] ; Bento, Ricardo Ferreira ^[3]

Affiliation

^[1] Univ Sao Paulo, Fac Med, Dept Otorhinolaryngol, BR-01231011 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Otorhinolaryngol, BR-09500900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Dept Otolaryngol, BR-09500900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-09500900 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med, Dept Surg, Div Plast Surg, BR-09500900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 27, n. 11, p. 747-750, NOV 2012.
• Web of Science Citations: 3

Abstract

PURPOSE: To establish a model to quantitative histological analysis of the mandibular branch of the facial nerve in rats. METHODS: Eleven Wistar rats had their right and left mandibular branches of the facial nerve surgically removed and were sacrificed afterwards. Quantitative histological analysis was performed with: a) partial number of axons; b) partial area of the transversal cut of the nerve (9000 mu m(2)); c) partial density. The averages of partial density were obtained. The statistical study was established by Wilcoxon test (p=0.05). RESULTS: In relation to density of axons, comparison between sides shows no statistically significant difference (p=0.248; p=0.533). Mean partial density of distal and proximal samples was, respectively, 0.18 +/- 0.02 and 0.19 +/- 0.02 axons/mu m(2). Comparison between proximal and distal samples shows no statistically significant difference (p=0.859; p=0.182). CONCLUSION: This study has successfully established a model to histological quantitative analysis of the mandibular branch of the facial nerve in rats. (AU)

• FAPESP's process: 08/00972-4 - Poliglicolic acid neurotube and bone marrow-derived cells differentiated in vitro in facial nerve regeneration: experimental study in rats
• Grantee: Ricardo Ferreira Bento
• Support Opportunities: Regular Research Grants