Evaluation of cytotoxic, genotoxic and antigenotoxic potential of Solanum lycocarpum fruits glicoalkaloid extract in V79 cells

Author(s)

Munari, Carla Carolina ^[1] ; de Oliveira, Pollyanna Francielli ^[1] ; de Souza Lima, Ildercilio Mota ^[1] ; Lima Martins, Sabrina de Paula ^[1] ; da Costa, Juliana de Carvalho ^[2] ; Bastos, Jairo Kenupp ^[2] ; Tavares, Denise Crispim ^[1]

Affiliation

^[1] Univ Franca, BR-14404600 Franca, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Food and Chemical Toxicology; v. 50, n. 10, p. 3696-3701, OCT 2012.
• Web of Science Citations: 15

Abstract

Solanum lycocarpum St.-Hil (Solanaceae) is a hairy shrub or small much-branched tree of the Brazilian Cerrado, popularly known as ``fruit-of-wolf{''}. Considering that the induction of chromosomal mutations is involved in the process of carcinogenesis, and that S. lycocatpum is often used in folk medicine, it becomes relevant to study its effect on genetic material. In this sense, the aim of present study was to determine the possible cytotoxic, genotoxic and antigenotoxic potentials of S. lycocarpum fruits glycoalkaloid extract (SL) in Chinese hamster lung fibroblasts (V79 cells). The cytotoxicity was evaluated by the colony forming assay, apoptosis and necrosis assay. Trypan blue exclusion dye method and mitotic index. Genotoxic and antigenotoxic potential were evaluated by comet and chromosomal aberrations assays. Four concentrations of SL (4, 8, 16 and 32 mu g/mL) were used for the evaluation of its genotoxic potential. The DNA damage-inducing agent methyl methanesulfonate (MMS, 221 mu g/mL) was utilized in combination with extract to evaluate a possible protective effect. The results showed that SL was cytotoxic at concentrations above 32 mu g/mL by the colony forming assay. For apoptosis and necrosis assay, the concentration of 64 mu g/mL of SL showed statistically significant increase in cell death by apoptosis and necrosis, while the concentrations of 128 and 256 mu g/mL of SL demonstrated statistically significant increase in cell death by necrosis, compared with the control group. Analysis of cell viability by Trypan blue exclusion indicated >96% viability for treatments with concentrations up to 32 mu g/mL of SL No significant differences in MI were observed between cultures treated with different concentrations of 51 (4, 8, 16 and 32 mu g/mL) alone or in combination with MMS and the negative control, indicating that these treatments were not cytotoxic. The comet and chromosomal aberrations assays revealed that SL does not display genotoxic activity. Moreover, the different concentrations of SL showed protective effect against both genomic and chromosomal damages induced by MMS. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/15871-1 - Evaluation of cytotoxic, antigenotoxic, antimutagenic and anticarcinogenic activities of Solanum lycocarpum and its majoritary compounds on in vitro and in vivo mammalian cells
• Grantee: Carla Carolina Munari
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/05732-4 - Study of cytotoxic and genotoxic potential of Solanum lycocarpum and their majoritary compounds and their influence on genotoxicity induced by different mutagens
• Grantee: Denise Crispim Tavares
• Support Opportunities: Regular Research Grants