| Full text | |
| Author(s): |
Moraes, Evandro M.
[1]
;
Perez, Manolo F.
[1]
;
Teo, Mariana F.
[1]
;
Zappi, Daniela C.
[2]
;
Taylor, Nigel P.
[3]
;
Machado, Marlon C.
[4]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Biol, BR-18052780 Sao Paulo - Brazil
[2] Royal Bot Gardens, Richmond TW9 3AB, Surrey - England
[3] Gardens Bay & Singapore Bot Gardens, Natl Pk Board, Singapore 259569 - Singapore
[4] Univ Estadual Feira de Santana, Dept Ciencias Biol, BR-44031460 Feira De Santana, BA - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Genetica; v. 140, n. 7-9, p. 277-285, SEP 2012. |
| Web of Science Citations: | 8 |
| Abstract | |
The Pilosocereus aurisetus group contains eight cactus species restricted to xeric habitats in eastern and central Brazil that have an archipelago-like distribution. In this study, 5-11 microsatellite markers previously designed for Pilosocereus machrisii were evaluated for cross-amplification and polymorphisms in ten populations from six species of the P. aurisetus group. The genotypic information was subsequently used to investigate the genetic relationships between the individuals, populations, and species analyzed. Only the Pmac101 locus failed to amplify in all of the six analyzed species, resulting in an 88 % success rate. The number of alleles per polymorphic locus ranged from 2 to 12, and the most successfully amplified loci showed at least one population with a larger number of alleles than were reported in the source species. The population relationships revealed clear genetic clustering in a neighbor-joining tree that was partially incongruent with the taxonomic limits between the P. aurisetus and P. machrisii species, a fact which parallels the problematic taxonomy of the P. aurisetus group. A Bayesian clustering analysis of the individual genotypes confirmed the observed taxonomic incongruence. These microsatellite markers provide a valuable resource for facilitating large-scale genetic studies on population structures, systematics and evolutionary history in this group. (AU) | |