| Full text | |
| Author(s): |
Quel, N. G.
[1]
;
Aragao, A. Z. B.
[1]
;
Salvadori, M. R.
[1]
;
Farias, A. S.
[1]
;
Joazeiro, P. P.
[2]
;
Santos, L. M. B.
[1]
;
Sa, L. R. M.
[3]
;
Ferreira, A. J. P.
[3]
;
Yano, T.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet Evolucao & Bioagentes, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Histol & Embriol, BR-13083862 Campinas, SP - Brazil
[3] Univ Sao Paulo, Dept Patol, Fac Med Vet & Zootecnia, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Veterinary Microbiology; v. 162, n. 2-4, p. 866-872, MAR 23 2013. |
| Web of Science Citations: | 1 |
| Abstract | |
Escherichia coli Vacuolating Factor (ECVF) is a heat-labile, vacuolating cytotoxin produced by avian pathogenic E. coli (APEC) isolated from avian cellulitis lesions. In this report, we intend to demonstrate that purified ECVF induces the inflammatory process of cellulitis. Our group is the first to demonstrate the effect of ECVF in a histological analysis by in situ inoculation of broiler chickens with purified ECVF. The animals were inoculated with the APEC AC53 and with purified ECVF subcutaneously on their ventral surface (in the sternum region). The histological analysis showed different grades of an acute inflammatory response in the epidermis, dermis and panniculus. An increase in mRNA expression of the proinflammatory cytokine TNF-alpha was also demonstrated in the inflamed tissue. When ECVF was systemically administered, increased levels of TNF-alpha and IL-10 were observed in the serum. These results suggest that ECVF plays a key role in the inflammatory process associated with cellulitis that is mainly mediated by TNF-alpha. In addition, this inflammation can be downregulated by the anti-inflammatory cytokine IL-10. (C) 2012 Published by Elsevier B.V. (AU) | |