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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polyketide Synthase Gene Diversity within the Microbiome of the Sponge Arenosclera brasiliensis, Endemic to the Southern Atlantic Ocean

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Author(s):
Trindade-Silva, Amaro E. [1, 2] ; Rua, Cintia P. J. [2] ; Andrade, Bruno G. N. [3] ; Paulo Vicente, Ana Carolina [3] ; Silva, Genivaldo G. Z. [4] ; Berlinck, Roberto G. S. [1] ; Thompson, Fabiano L. [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biol, Rio De Janeiro - Brazil
[3] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Rio De Janeiro - Brazil
[4] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 - USA
Total Affiliations: 4
Document type: Journal article
Source: Applied and Environmental Microbiology; v. 79, n. 5, p. 1598-1605, MAR 2013.
Web of Science Citations: 14
Abstract

Microbes associated with marine sponges are considered important producers of bioactive, structurally unique polyketides. The synthesis of such secondary metabolites involves type I polyketide synthases (PKSs), which are enzymes that reach a maximum complexity degree in bacteria. The Haplosclerida sponge Arenosclera brasiliensis hosts a complex microbiota and is the source of arenosclerins, alkaloids with cytotoxic and antibacterial activity. In the present investigation, we performed high-throughput sequencing of the ketosynthase (KS) amplicon to investigate the diversity of PKS genes present in the metagenome of A. brasiliensis. Almost 4,000 ketosynthase reads were recovered, with about 90% annotated automatically as bacterial. A total of 235 bacterial KS contigs was rigorously assembled from this sequence pool and submitted to phylogenetic analysis. A great diversity of six type I PKS groups has been consistently detected in our phylogenetic reconstructions, including a novel and A. brasiliensis-exclusive group. Our study is the first to reveal the diversity of type I PKS genes in A. brasiliensis as well as the potential of its microbiome to serve as a source of new polyketides. (AU)

FAPESP's process: 10/50190-2 - Investigation of metabolic and biotechnological potential of marine organisms in bioremediation processes and for the production of substances with anti-viral, anti-inflammatory and anti-Leishmania activities (Marine Biodiversity)
Grantee:Roberto Gomes de Souza Berlinck
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 09/11612-1 - Characterization of the biosynthetic gene cluster of Arenosclerins, alkylpiperidine alkaloids isolated from the sponge Arenosclera brasiliensis
Grantee:Amaro Emiliano Trindade Silva
Support type: Scholarships in Brazil - Post-Doctorate