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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Nature-Inspired Betalainic Probe for Live-Cell Imaging of Plasmodium-Infected Erythrocytes

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Pires Goncalves, Leticia Christina [1] ; Tonelli, Renata Rosito [2] ; Bagnaresi, Piero [3] ; Mortara, Renato Arruda [4] ; Ferreira, Antonio Gilberto [5] ; Bastos, Erick Leite [6]
Total Authors: 6
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Ciencias Biol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunobiol & Parasitol, Sao Paulo - Brazil
[5] Univ Fed Sao Carlos, Dept Quim, Sao Paulo - Brazil
[6] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: PLoS One; v. 8, n. 1 JAN 16 2013.
Web of Science Citations: 17

A model betalainic dye was semisynthesized from betanin, the magenta pigment of the red beet, and was effective for live-cell imaging of Plasmodium-infected red blood cells. This water-soluble fluorescent probe is photostable, excitable in the visible region and cell membrane-permeable, and its photophysical properties are not notably pH-sensitive. Fluorescence imaging microscopy of erythrocytes infected with Plasmodium falciparum, a causative agent of malaria in humans, showed that only the parasite was stained. Z-stacking analysis suggested that the probe accumulates proximal to the nucleus of the parasite. Indicaxanthin, one of the natural fluorescent betalains found in the petals of certain flowers, did not stain the parasite or the red blood cell. (AU)

FAPESP's process: 07/59407-1 - Semi-synthetic betalains as biocompatible antioxidants and pigments
Grantee:Leticia Christina Pires Gonçalves
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 07/00684-6 - Synthesis and application of modified flower pigments
Grantee:Erick Leite Bastos
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 11/23036-5 - Floral betalamic pigments: fluorescence and antiradical capacity
Grantee:Erick Leite Bastos
Support type: Regular Research Grants
FAPESP's process: 10/15042-2 - Infection by Giardia lamblia: search for molecules involved in parasita-host interaction
Grantee:Renata Rosito Tonelli
Support type: Research Grants - Young Investigators Grants