| Full text | |
| Author(s): |
de Lima, Flavia Mafra
[1]
;
Albertini, Regiane
[1]
;
Dantas, Yvana
[2]
;
Maia-Filho, Antonio Luis
[3]
;
Santana, Cristiano de Loura
[4]
;
Castro-Faria-Neto, Hugo Caire
[5]
;
Franca, Cristiane
[4]
;
Villaverde, Antonio Balbin
[6]
;
Aimbire, Flavio
[7]
Total Authors: 9
|
| Affiliation: | [1] Univ Nove Julho UNINOVE, Dept Rehabil Sci, Sao Paulo - Brazil
[2] Intes Care Unity Hosp & Matern, Teresina, PI - Brazil
[3] Fac Integral Diferencial FACID, Teresina, PI - Brazil
[4] Univ Nove Julho UNINOVE, Res Grp Biophoton, Sao Paulo - Brazil
[5] Fiocruz MS, Lab Immunopharmacol, IOC, BR-21045900 Rio De Janeiro, RJ - Brazil
[6] UNICASTELO, Inst Biomed Engn, Sao Jose Dos Campos, SP - Brazil
[7] Fed Univ Sao Paulo UNIFESP, Dept Sci & Technol, Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Photochemistry and Photobiology; v. 89, n. 1, p. 179-188, JAN-FEB 2013. |
| Web of Science Citations: | 31 |
| Abstract | |
It remains unknown if the oxidative stress can be regulated by low-level laser therapy (LLLT) in lung inflammation induced by intestinal reperfusion (i-I/R). A study was developed in which rats were irradiated (660 nm, 30 mW, 5.4 J) on the skin over the bronchus and euthanized 2 h after the initial of intestinal reperfusion. Lung edema and bronchoalveolar lavage fluid neutrophils were measured by the Evans blue extravasation and myeloperoxidase (MPO) activity respectively. Lung histology was used for analyzing the injury score. Reactive oxygen species (ROS) was measured by fluorescence. Both expression intercellular adhesion molecule 1 (ICAM-1) and peroxisome proliferator-activated receptor-y (PPARy) were measured by RT-PCR. The lung immunohistochemical localization of ICAM-1 was visualized as a brown stain. Both lung HSP70 and glutathione protein were evaluated by ELISA. LLLT reduced neatly the edema, neutrophils influx, MPO activity and ICAM-1 mRNA expression. LLLT also reduced the ROS formation and oppositely increased GSH concentration in lung from i-I/R groups. Both HSP70 and PPARy expression also were elevated after laser irradiation. Results indicate that laser effect in attenuating the acute lung inflammation is driven to restore the balance between the pro- and antioxidants mediators rising of PPARy expression and consequently the HSP70 production. (AU) | |
| FAPESP's process: | 08/08838-5 - Study of anti-inflammatory action mechanism of Low Level Laser on experimental model of allergic and systemic pulmonary inflammation. |
| Grantee: | Flávio Aimbire Soares de Carvalho |
| Support Opportunities: | Regular Research Grants |