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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of Staphylococcus saprophyticus isolated from patients with urinary tract infection using a simple set of biochemical tests correlating with 16S-23S interspace region molecular weight patterns

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Author(s):
Ferreira, Adriano Martison [1, 2] ; Bonesso, Mariana Favero [1, 2] ; Mondelli, Alessandro Lia [3] ; Ribeiro de Souza da Cunha, Maria de Lourdes [1]
Total Authors: 4
Affiliation:
[1] UNESP Univ Estadual Paulista, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[2] UNESP Univ Estadual Paulista, Univ Hosp, Botucatu Sch Med, Dept Trop Dis, Botucatu, SP - Brazil
[3] UNESP Univ Estadual Paulista, Univ Hosp, Botucatu Sch Med, Dept Internal Med, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Microbiological Methods; v. 91, n. 3, p. 406-411, DEC 2012.
Web of Science Citations: 11
Abstract

The emergence of Staphylococcus spp. not only as human pathogens, but also as reservoirs of antibiotic resistance determinants, requires the development of methods for their rapid and reliable identification in medically important samples. The aim of this study was to compare three phenotypic methods for the identification of Staphylococcus spp. isolated from patients with urinary tract infection using the PCR of the 16S-23S interspace region generating molecular weight patterns (ITR-PCR) as reference. All 57 S. saprophyticus studied were correctly identified using only the novobiocin disk. A rate of agreement of 98.0% was obtained for the simplified battery of biochemical tests in relation to ITR-PCR, whereas the Vitek I system and novobiocin disk showed 81.2% and 89.1% agreement, respectively. No other novobiocin-resistant non-S. saprophyticus strain was identified. Thus, the novobiocin disk is a feasible alternative for the identification of S. saprophyticus in urine samples in laboratories with limited resources. ITR-PCR and the simplified battery of biochemical tests were more reliable than the commercial systems currently available. This study confirms that automated systems are still unable to correctly differentiate CoNS species and that simple, reliable and inexpensive methods can be used for routine identification. (c) 2012 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/10083-5 - Comparison of methods from identification and profile of sensitivity to antimicrobial in Staphylococcus spp. isolated from patients with urinary tract infection (UTI)
Grantee:Maria de Lourdes Ribeiro de Souza da Cunha
Support Opportunities: Regular Research Grants