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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Experimental infection of suckling mice by subcutaneous inoculation with Oropouche virus

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Santos, Rodrigo I. [1] ; Almeida, Mariana F. P. [1] ; Paula, Flavia E. [1] ; Rodrigues, Alcir Humberto [1] ; Saranzo, Ariane Mattioli [1] ; Paula, Andre E. [1] ; Silva, Maria Lucia [1] ; Alves Correa, Vani Maria [1] ; Acrani, Gustavo Olszanski [1] ; Neder, Luciano [2] ; Arruda, Eurico [1]
Total Authors: 11
[1] Univ Sao Paulo, Dept Cell Biol, Sch Med, BR-14049 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Dept Pathol, Sch Med, BR-14049 Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: VIRUS RESEARCH; v. 170, n. 1-2, p. 25-33, DEC 2012.
Web of Science Citations: 11

Oropouche virus, of the family Bunyaviridae, genus Orthobunyavirus, serogroup Simbu, is an important causative agent of arboviral febrile illness in Brazil. An estimated 500,000 cases of Oropouche fever have occurred in Brazil in the last 30 years, with recorded cases also in Panama, Peru, Suriname and Trinidad. We have developed an experimental model of Oropouche virus infection in neonatal BALB/c mouse by subcutaneous inoculation. The vast majority of infected animals developed disease on the 5th day post infection, characterized mainly by lethargy and paralysis, progressing to death within 10 days. Viral replication was documented in brain cells by in situ hybridization, immunohistochemistry and virus titration. Multi-step immunohistochemistry indicated neurons as the main target cells of OROV infection. Histopathology revealed glial reaction and astrocyte activation in the brain and spinal cord, with neuronal apoptosis. Spleen hyperplasia and mild meningitis were also found, without viable virus detected in liver and spleen. This is the first report of an experimental mouse model of OROV infection, with severe involvement of the central nervous system, and should become useful in pathogenesis studies, as well as in preclinical testing of therapeutic interventions for this emerging pathogen. (c) 2012 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 08/50617-6 - Studies on emerging viruses including arbovirus, robovirus, respiratory viruses and congenital transmission, at the Centro de Pesquisa em Virologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo
Grantee:Luiz Tadeu Moraes Figueiredo
Support type: Research Projects - Thematic Grants