Oral tolerance and OVA-induced tolerogenic dendritic cells reduce the severity of collagen/ovalbumin-induced arthritis in mice

Author(s)

Thome, Rodolfo ^[1] ; Romani Fernandes, Luis Gustavo ^[1] ; Mineiro, Marcela Franco ^[1] ; Simioni, Patricia Ucelli ^[1] ; Joazeiro, Paulo Pinto ^[2] ; da Silva Cunha Tamashiro, Wirla Maria ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Genet Evolut & Bioagents, Inst Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Histol & Embryol, Inst Biol, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Cellular Immunology; v. 280, n. 1, p. 113-123, NOV 2012.
• Web of Science Citations: 13

Abstract

Dietary proteins play an important role in the regulation of systemic immune response, in a phenomenon known as oral tolerance (OT). To evaluate the effects of OT on a murine model of type II collagen (CII) plus ovalbumin (OVA)-induced arthritis (CIA), mice were fed with OVA either before or after CIA induction. OT significantly reduced the paw edema and synovial inflammation, as well as serum levels of anti-CII, the ex vivo proliferation and inflammatory cytokine production by spleen cells from CIA mice. The frequencies of Foxp3(+) and IL-10(+) cells were higher, whereas IFN gamma(+), cells and IL-17(+) cells were lower, among gated CD4(+) spleen T cells from tolerized CIA mice than in those from non-tolerized CIA mice. Adoptive transfer of tolerogenic dendritic cells (DCs) before CIA induction mimics the effects observed in the OT. We demonstrate here that bystander suppression induced by OT can modify the course of CIA and tolerogenic DCs play a role this phenomenon. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 09/05891-5 - Experimental arthritis induced in mice by collagen type II associated with ovalbumin: modulation by oral tolerance
• Grantee: Wirla Maria da Silva Cunha Tamashiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/01838-2 - Modulation of experimental arthritis induced by the association of ovalbumin and type II collagen
• Grantee: Rodolfo Thomé
• Support Opportunities: Scholarships in Brazil - Master