Study of C- and O-glycosylflavones in sugarcane extracts using liquid chromatography: exact mass measurement mass spectrometry

Full text
Author(s):	Renata Colombo ^[1] ; Janete H. Yariwake ^[2] ; Michael McCullagh ^[3] Total Authors: 3
Affiliation:	^[1] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil ^[2] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil ^[3] Waters Corporation - Ucrânia Total Affiliations: 3
Document type:	Journal article
Source:	Journal of the Brazilian Chemical Society; v. 19, n. 3, p. 483-490, 2008-00-00.
Abstract
The flavonoids present in sugarcane (Saccharum officinarum) extracts were analyzed by liquid chromatography - mass spectrometry (LC-MS), and a study of the fragmentation patterns of selected flavonoids was conducted using orthogonal acceleration time-of-flight electrospray ionization mass spectrometry (ESI-oa-ToF MS). Seven C- and O-glycosylflavones were identified in the extracts, namely, schaftoside, isoschaftoside, luteolin-8-C-(rhamnosylglucoside), vitexin, orientin, tricin-7-O-neohesperidoside and tricin-7-O-glucoside. Of these, five were identified in the absence of direct comparison with their respective standards. The described method also permitted the differentiation of the 6-C and 8-C isomeric flavones, schaftoside and isoschaftoside. The combination of fragmentation data and exact mass measurement showed to be complimentary to the HPLC-UV-MS techniques previously utilized for isomers discrimination in sugarcane studies. (AU)