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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden

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Andréia Bartachini Gomes ; Killarney Ataíde Soares ; Ednéia Casagrande Bueno ; Noeli Maria Espindola ; Alberto Hiroshi Iha ; Antônio Augusto Mendes Maia ; Regina Helena Saramargo Peralta ; Adelaide José Vaz
Total Authors: 8
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 102, n. 6, p. 725-731, Set. 2007.

Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs. (AU)

FAPESP's process: 02/12061-0 - Cysticercosis: immunidiagnosis, antigens, immune response, clinical and epidemiologic aspects
Grantee:Adelaide José Vaz
Support type: Research Projects - Thematic Grants