| Full text | |
| Author(s): |
Gastalho Campos, Luciene Cristina
[1]
;
Miyakawa, Ayumi Aurea
[1]
;
Barauna, Valerio Garrone
[1]
;
Cardoso, Leandro
[1]
;
Borin, Thaiz Ferraz
[1]
;
de Oliveira Dallan, Luis Alberto
[1]
;
Krieger, Jose Eduardo
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med, Heart Inst InCor, Lab Genet & Mol Cardiol, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Cardiovascular Research; v. 83, n. 1, p. 140-147, July 2009. |
| Field of knowledge: | Biological Sciences - Physiology |
| Web of Science Citations: | 14 |
| Abstract | |
Cysteine- and glycine-rich protein 3/muscle LIM-domain protein (CRP3/MLP) mediates protein-protein interaction with actin filaments in the heart and is involved in muscle differentiation and vascular remodelling. Here, we assessed the induction of CRP3/MLP expression during arterialization in human and rat veins. Vascular CRP3/MLP expression was mainly observed in arterial samples from both human and rat. Using quantitative real time RT-PCR, we demonstrated that the CRP3/MLP expression was 10 times higher in smooth muscle cells (SMCs) from human mammary artery (h-MA) vs. saphenous vein (h-SV). In endothelial cells (ECs), CRP3/MLP was scarcely detected in either h-MA or h-SV. Using an ex vivo flow through system that mimics arterial condition, we observed induction of CRP3/MLP expression in arterialized h-SV. Interestingly, the upregulation of CRP3/MLP was primarily dependent on stretch stimulus in SMCs, rather than shear stress in ECs. Finally, using a rat vein in vivo arterialization model, early (1-14 days) CRP3/MLP immunostaining was observed predominantly in the inner layer and later (28-90 days) it appeared more scattered in the vessel layers. Here we provide evidence that CRP3/MLP is primarily expressed in arterial SMCs and that stretch is the main stimulus for CRP3/MLP induction in veins exposed to arterial haemodynamic conditions. (AU) | |
| FAPESP's process: | 01/00009-0 - An integrated approach for the dissection of primary hypertension: molecular and functional characterization of the cardiovascular system |
| Grantee: | Eduardo Moacyr Krieger |
| Support Opportunities: | Research Projects - Thematic Grants |