Oligofructose supplementation (10%) during pregnancy and lactation does not change the inflammatory effect of concurrent trans fatty acid ingestion on 21-day-old offspring

Author(s)

Losinskas Hachul, Ana Claudia ^[1] ; Mennitti, Lais Vales ^[2] ; de Oliveira, Juliana Lopes ^[1] ; Moreno, Mayara Franzoi ^[1] ; Okuda, Marcos Hiromu ^[1] ; dos Santos, Bruno ^[1] ; Oyama, Lila Missae ^[1] ; Ribeiro, Eliane Beraldi ^[1] ; Oller do Nascimento, Claudia Maria ^[1] ; Pisani, Luciana Pellegrini ^[2]

Affiliation

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Disciplina Fisiol Nutr, Dept Fis, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Inst Saude & Sociedade, Dept Biociencias, Santos, SP - Brazil

• Document type: Journal article
• Source: LIPIDS IN HEALTH AND DISEASE; v. 12, MAY 1 2013.
• Web of Science Citations: 7

Abstract

Background: Previously, we demonstrated that trans fatty acid ingestion during pregnancy and lactation caused a pro-inflammatory effect on the newborn. The opposite effect was described for gestational prebiotic intake. In the present study, we examined whether supplementation of the diet of the dams with 10% of oligofructose with or without hydrogenated vegetable fat during pregnancy and lactation affected the pro-inflammatory status on the pups at age 21 days. Methods: On the first day of pregnancy, rats were divided into four groups, each of which received one of four diets: a control diet (C group), a control diet supplemented with 10% oligofructose (CF group), a diet enriched with hydrogenated vegetable fat containing trans fatty acids (T group) or a diet enriched with hydrogenated vegetable fat containing trans fatty acids supplemented with 10% oligofructose (TF group). The pups were weighed at birth and at 7, 14 and 21 days of life and were euthanized on post-natal day 21. The serum glucose, insulin and adiponectin concentrations were analyzed. The IL-6, IL-10 and TNF-alpha contents of the retroperitoneal white adipose tissue, liver, soleus and extensor digital longus muscles were analyzed by ELISA. The results are presented as the means +/- standard error of the mean. Statistical significance was assessed using two-way ANOVA, followed by Tukey's test and considered significant at p < 0.05 Results: The body weights of the 21-day old pups in the CF and TF groups were significant lower than those of the C (27% and 21%) and T (25% and 19%, respectively) groups. The serum levels of adiponectin in the CF, T and TF groups were lower than in the C group (41%; 34% and 31%, respectively). In the retroperitoneal adipose tissue, the IL-6 content was increased in TF group relative to the C and CF groups (74% for both), and the TNF-alpha content was higher in the T and TF groups than in the C group (62% and 98%, respectively). In the liver, the TNF-alpha (56% and 104%) and IL-10 (52% and 73%) contents were increased in the CF group relative to the C and TF groups. Conclusions: Supplementation of the diet of the dams with 10% of oligofructose during pregnancy and lactation, independent of supplementation with hydrogenated vegetable fat, adversely affected the development of the offspring and contributed to development of a pro-inflammatory status in the pups on postnatal day 21. (AU)

• FAPESP's process: 11/19426-2 - Effect of diet enriched with prebiotics in the intracellular mechanism involved with development of insulin resistance in offspring, 21 and 90 days of life, of mothers treated with trans fatty acids, during pregnancy and lactation
• Grantee: Luciana Pellegrini Pisani
• Support Opportunities: Regular Research Grants