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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of zoledronic acid on odontoblast-like cells

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Author(s):
Basso, F. G. [1] ; Turrioni, A. P. S. [2] ; Hebling, J. [2] ; de Souza Costa, C. A. [2]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 58, n. 5, p. 467-473, MAY 2013.
Web of Science Citations: 13
Abstract

The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 x 10(4) cells/cm(2)) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 mu M) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability - MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five mu M ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 mu M ZOL caused a significant increase in Col-I expression. Although 5 mu M ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 09/54722-1 - In vitro evaluation of the effects of low level laser therapy on cultured osteoblasts, fibroblasts and keratonicytes submitted to zoledronic acid therapy
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants