Effects of elevated CO2 concentration and water deficit on fructan metabolism in Viguiera discolor Baker

Author(s)

Oliveira, V. F. ^[1] ; Silva, E. A. ^[1] ; Zaidan, L. B. P. ^[1] ; Carvalho, M. A. M. ^[1]

Affiliation

^[1] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, BR-04045972 Sao Paulo - Brazil

• Document type: Journal article
• Source: Plant Biology; v. 15, n. 3, p. 471-482, MAY 2013.
• Web of Science Citations: 15

Abstract

Elevated {[}CO2] is suggested to mitigate the negative effects of water stress in plants; however responses vary among species. Fructans are recognised as protective compounds against drought and other stresses, as well as having a role as reserve carbohydrates. We analysed the combined effects of elevated {[}CO2] and water deficit on fructan metabolism in the Cerrado species Viguiera discolor Baker. Plants were cultivated for 18days in open-top chambers (OTC) under ambient (approximate to 380ppm), and high (approximate to 760ppm) {[}CO2]. In each OTC, plants were submitted to three treatments: (i) daily watering (control), (ii) withholding water (WS) for 18days and (iii) re-watering (RW) on day 11. Analyses were performed at time 0 and days 5, 8, 11, 15 and 18. High {[}CO2] increased photosynthesis in control plants and increased water use efficiency in WS plants. The decline in soil water content was more distinct in WS 760 (WS under 760ppm), although the leaf and tuberous root water status was similar to WS 380 plants (WS under 380ppm). Regarding fructan active enzymes, 1-SST activity decreased in WS plants in both CO2 concentrations, a result consistent with the decline in photosynthesis and, consequently, in substrate availability. Under WS and both {[}CO2] treatments, 1-FFT and 1-FEH seemed to act in combination to generate osmotically active compounds and thus overcome water deficit. The proportion of hexoses to sucrose, 1-kestose and nystose (SKN) was higher in WS plants. In WS 760, this increase was higher than in WS 380, and was not accompanied by decreases in SKN at the beginning of the treatment, as observed in WS 380 plants. These results suggest that the higher {[}CO2] in the atmosphere contributed to maintain, for a longer period, the pool of hexoses and of low DP fructans, favouring the maintenance of the water status and plant survival under drought. (AU)

• FAPESP's process: 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers
• Grantee: Marcia Regina Braga
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 07/59782-7 - Interaction between the atmosphere enriched in CO2 and the water deficit: effects on the growth and the metabolism of fructans in Vernonia herbaceae (Vell.) Rusby
• Grantee: Vanessa Fátima de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate