Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quadriceps Muscle Atrophy After Anterior Cruciate Ligament Transection Involves Increased mRNA Levels of Atrogin-1, Muscle Ring Finger 1, and Myostatin

Full text
Author(s):
Delfino, Gabriel B. [1] ; Peviani, Sabrina M. [1] ; Durigan, Joao L. Q. [1] ; Russo, Thiago L. [1] ; Baptista, Igor L. [2] ; Ferretti, Mario [3] ; Moriscot, Anselmo S. [2] ; Salvini, Tania F. [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos, Dept Phys Therapy, BR-13565905 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Orthoaped Surg, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION; v. 92, n. 5, p. 411-419, MAY 2013.
Web of Science Citations: 12
Abstract

Delfino GB, Peviani SM, Durigan JLQ, Russo TL, Baptista IL, Ferretti M, Moriscot AS, Salvini TF: Quadriceps muscle atrophy after anterior cruciate ligament transection involves increased mRNA levels of atrogin-1, muscle ring finger 1, and myostatin. Am J Phys Med Rehabil 2013;92:411-419. Objective: The aim of this study was to assess the mRNA levels of atrogin-1, muscle ring finger 1, and myostatin in rat quadriceps after anterior cruciate ligament (ACL) transection. Design: Wistar rats were randomized into three different groups: ACL (surgery and ACL transection), sham (surgery without ACL transection), and control. Vastus medialis, rectus femoris, and vastus lateralis muscles were harvested at 1, 2, 3, 7, and 15 days after ACL transection. The mRNA levels of atrogin-1, muscle ring finger 1, and myostatin, as well as the ubiquitinated protein content, muscle mass, and cross-sectional area of the muscle fibers, were evaluated. Results: Elevated levels of atrogin-1, muscle ring finger 1, and myostatin mRNA were detected in all tested muscles at most time points. The ubiquitinated protein content was increased at 3 days in the ACL and sham groups. The muscle mass of the ACL group was reduced at 3, 7, and 15 days (vastus lateralis and vastus medialis) and at 7 and 15 days (rectus femoris), whereas it was reduced in the sham group at 3 and 7 days (vastus lateralis and vastus medialis) and at 7 days (rectus femoris). The cross-sectional area of vastus medialis was reduced at 3, 7, and 15 days in the ACL group and at 3 and 7 days in the sham group. The cross-sectional area of the vastus lateralis was reduced at 7 and 15 days in the ACL group and at 7 days in the sham group. Whereas muscle mass and cross-sectional area recovery was noted in the sham group, no recovery was observed in the ACL group. Conclusions: Quadriceps atrophy after ACL transection involves increased levels of myostatin, atrogin-1, and muscle ring finger 1 mRNA and the accumulation of ubiquitinated protein. (AU)

FAPESP's process: 08/09408-4 - The effect of electric stimulation in quadriceps femoris muscle atrophy after anterior cruciate ligament injury in rats
Grantee:João Luiz Quagliotti Durigan
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 08/03499-8 - The effect of anterior cruciate ligament rupture in morphology, gene and protein expressions related to atrophy in the quadriceps femoral muscle of rats
Grantee:Gabriel Borges Delfino
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 10/06940-7 - Effect of anterior cruciate ligament rupture on the expression of genes and proteins related to atrophy in quadriceps muscle of rats
Grantee:Tania de Fatima Salvini
Support Opportunities: Regular Research Grants