| Full text | |
| Author(s): |
Manzine, Livia Regina
[1]
;
Balasco Serrao, Vitor Hugo
[1]
;
Trambaioli da Rocha e Lima, Luis Mauricio
[2, 3]
;
de Souza, Marcos Michel
[1]
;
Bettini, Jefferson
[4]
;
Portugal, Rodrigo Villares
[4]
;
van Heel, Marin
[5, 6]
;
Thiemann, Otavio Henrique
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Phys Sao Carlos, Dept Phys & Informat, BR-13560970 Sao Carlos, SP - Brazil
[2] Brazilian Natl Inst Metrol Qual & Technol INMETRO, Lab Biotechnol LaBio DIPRO, Rio De Janeiro - Brazil
[3] Univ Fed Rio de Janeiro, Natl Inst Sci & Technol Struct Biol & Bioimaging, BR-21941590 Rio De Janeiro - Brazil
[4] Ctr Nacl Pesquisa Energia Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil
[5] Univ London Imperial Coll Sci Technol & Med, London SW7 2AZ - England
[6] Leiden Univ, Gorlaeus Labs, Leiden Inst Chem, NL-9502 Leiden - Netherlands
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | FEBS Letters; v. 587, n. 7, p. 906-911, APR 2 2013. |
| Web of Science Citations: | 4 |
| Abstract | |
In bacteria selenocysteyl-tRNA(sec) (SelC) is synthesized by selenocysteine synthase (SelA). Here we show by fluorescence anisotropy binding assays and electron microscopical symmetry analysis that the SelA-tRNA(sec) binding stoichiometry is of one tRNA(sec) molecule per SelA monomer (1: 1) rather than the 1: 2 value proposed previously. Negative stain transmission electron microscopy revealed a D5 pointgroup symmetry for the SelA-tRNA(sec) assembly both with and without tRNA(sec) bound. Furthermore, SelA can associate forming a supramolecular complex of stacked decamer rings, which does not occur in the presence of tRNA(sec). We discuss the structure-function relationships of these assemblies and their regulatory role in bacterial selenocysteyl-tRNA(sec) synthesis. (C) 2013 Published by Elsevier B. V. on behalf of the Federation of European Biochemical Societies. (AU) | |
| FAPESP's process: | 98/14138-2 - Center for Structural Molecular Biotechnology |
| Grantee: | Glaucius Oliva |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |