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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis induces long-term protection in presence of regulatory T cells

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Author(s):
de Amorim, Juliana [1] ; Magalhaes, Adriana [1] ; Munoz, Julian Esteban [1] ; Rittner, Glauce M. G. [1] ; Nosanchuk, Joshua D. [2, 3] ; Travassos, Luiz R. [4] ; Taborda, Carlos P. [1, 5]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 - USA
[3] Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 - USA
[4] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
[5] Univ Sao Paulo, Lab Med Mycol IMT SP LIM53, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Microbes and Infection; v. 15, n. 3, p. 181-191, MAR 2013.
Web of Science Citations: 7
Abstract

Paracoccidioidomycosis is a granulomatous systemic mycosis endemic in Brazil and other Latin America countries. A DNA vaccine encoding the inununoprotective peptide 10 (P10) significantly reduced the fungal burden in mice when given prior to or after intratracheal challenge with Paracoccidioides brasiliensis. Presently, the generation/expansion of CD4(+) CD44(hi) memory T cells as well as Foxp3(+) Treg cells in mice immunized with the DNA vaccine (pcDNA3-P10) before and after infection with P. brasiliensis was investigated. Memory CD4(+) CD44(hi) T cells simultaneously with Foxp3(+) Treg cells increased in the spleens and lungs of pcDNA3-P10 immunized mice on day 0, 30, 60 and 120 postinfection. Histopathology of the lung tissue showed minimal inflammation in immunized mice compared with the unimmunized group, suggesting a role for regulatory T cells in controlling the immunopathology. The DNA vaccine shows that the repeated immunization generates memory cells and regulatory T cells that replace the initially protective pro-inflammatory T cells conferring a long term protection while preserving the integrity of the infected tissue. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

FAPESP's process: 11/17267-4 - Evaluation of a vaccine based on peptide-P10 and the therapeutic effect when administrate in combination with antifungal drugs in the fibrose experimental murine model induzed with Paracoccodioides brasiliensis conidia
Grantee:Carlos Pelleschi Taborda
Support Opportunities: Regular Research Grants
FAPESP's process: 09/15823-7 - Use of dendritic cells from mice and human pulsed with pVAX vector containing insert for the expression of P10 or P10 synthetic peptide derived from the GP 43 of P. brasiliensis, as vaccine strategy against paracoccidioidomycosis
Grantee:Carlos Pelleschi Taborda
Support Opportunities: Regular Research Grants
FAPESP's process: 10/51423-0 - Bioactive peptides and peptidases: biological and immunobiological activities in infectious diseases and cancer
Grantee:Luiz Rodolpho Raja Gabaglia Travassos
Support Opportunities: Research Projects - Thematic Grants