Activation profile of CXCL8-stimulated neutrophils and aging

Author(s)

Dalboni, Thalita Marcato ^{[1, 2]} ; Abe, Aneli Eiko ^{[1, 2]} ; de Oliveira, Carine Ervolino ^{[1, 3]} ; Lara, Vanessa Soares ^{[1, 3]} ; Campanelli, Ana Paula ^{[1, 2]} ; Gasparoto, Carlos Teodoro ^{[4, 5]} ; Gasparoto, Thais Helena ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol Oral Pathol, Bauru, SP - Brazil

^[4] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-05508 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Publ Hlth, Dept Publ Hlth, BR-05508 Sao Paulo - Brazil

• Document type: Journal article
• Source: CYTOKINE; v. 61, n. 3, p. 716-719, MAR 2013.
• Web of Science Citations: 13

Abstract

Neutrophils are pivotal effector cells of innate immunity representing the first line of defense against aggression. They are the first cells to arrive at the site of the aggression, where they can directly eliminate the invading microorganisms. Their activation and recruitment into peripheral tissues is indispensable for host defense. With aging, there are alterations of the receptor by driven functions of human neutrophils as a decrease in the functional changes in signaling elicited by specific receptors, as CXCR1. We investigated the activation of neutrophils from elderly after the cells were cultivated with CXCL8. Although, CXCL8 induced elastase (ELA) secretion, data showed neither myeloperoxidase (MPO) activity nor production of IL-6, IL-10, GM-CSF by neutrophils from elderly compared with young individuals. On the other hand, in the presence of only LPS or LPS associated with CXCL8 neutrophils from elderly individuals, there were significant levels of IL-6, IL-10, GM-CSF but not MPO. These results indicate that neutrophils from elderly do not respond to CXCL8 stimulus, but they are activated by LPS to produce cytokines. However, MPO activity from elderly individuals was not different in the presence or absence of LPS and CXCL8. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 10/19317-6 - Activation profile of neutrophils from elderly individuals and expression of IgG receptors
• Grantee: Thalita Marcato Dalboni
• Support Opportunities: Scholarships in Brazil - Scientific Initiation