Biostimulatory effect of low-level laser therapy on keratinocytes in vitro

Author(s)

Basso, Fernanda G. ^[1] ; Oliveira, Camila F. ^[2] ; Kurachi, Cristina ^[3] ; Hebling, Josimeri ^[2] ; de Souza Costa, Carlos A. ^{[2, 4]}

Affiliation

^[1] UNICAMP Univ Campinas, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[4] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, BR-14801903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 28, n. 2, p. 367-374, FEB 2013.
• Web of Science Citations: 63

Abstract

Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototype (LASERTable; 780 +/- 3 nm; 40 mW) using 0.5, 1.5, 3, 5, and 7 J/cm(2) energy doses. Irradiations were done every 24 h totaling three applications. Evaluation of cell metabolism (MTT assay) showed that LLLT with all energy doses promoted an increase of cell metabolism, being more effective for 0.5, 1.5, and 3 J/cm(2). The highest cell counts (Trypan blue assay) were observed with 0.5, 3, and 5 J/cm(2). No statistically significant difference for total protein (TP) production was observed and cell morphology analysis by scanning electron microscopy revealed that LLLT did not promote morphological alterations on the keratinocytes. Real-time polymerase chain reaction (qPCR) revealed that LLLT also promoted an increase of type I collagen (Col-I) and vascular endothelial growth factor (VEGF) gene expression, especially for 1.5 J/cm(2), but no change on fibroblast growth factor-2 (FGF-2) expression was observed. LLLT at energy doses ranging from 0.5 to 3 J/cm(2) promoted the most significant biostimulatory effects on cultured keratinocytes. (AU)

• FAPESP's process: 09/54722-1 - In vitro evaluation of the effects of low level laser therapy on cultured osteoblasts, fibroblasts and keratonicytes submitted to zoledronic acid therapy
• Grantee: Carlos Alberto de Souza Costa
• Support Opportunities: Regular Research Grants