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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microsatellite markers for genetic studies of the fall armyworm, Spodoptera frugiperda

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Pavinato, V. A. C. [1] ; Martinelli, S. [2] ; de Lima, P. F. [3] ; Zucchi, M. I. [4] ; Omoto, C. [1]
Total Authors: 5
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Entomol & Acarol, Lab Resistencia Insetos Taticas Controle, Piracicaba, SP - Brazil
[2] Monsanto Brasil Ltda, Sao Paulo - Brazil
[3] Inst Agron Campinas, Ctr Pesquisa & Desenvolvimento Recursos Genet Veg, Campinas, SP - Brazil
[4] Agencia Paulista Tecnol Agronegocios, Piracicaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Genetics and Molecular Research; v. 12, n. 1, p. 370-380, 2013.
Web of Science Citations: 3

We developed six microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The SSR loci were isolated with enriched genomic library protocol by using native individuals as a genome source for markers. These loci were characterized in 48 individuals and they were tested for the ability to identify candidate migrants exchanged among the samples. The number of alleles per locus ranged from 5 to 18 (10.8 on average). The observed polymorphism information content ranged from 0.172 to 0.891. Beside the lower efficiency to obtain SSR loci, the six microsatellites were polymorphic and sufficiently discriminant for the genetic studies of S. frugiperda; it allowed us to identify migrants with both NJ clustering and the Bayesian methods. These markers will be useful for molecular ecology studies of this highly polyphagous species in order to understand the processes that determine genetic differentiation in the complex agro-ecosystems that it infests and improve local integrated pest management practices. (AU)

FAPESP's process: 06/03373-9 - Susceptibility to Cry1Ac toxin and genetic structure in Alabama argillacea (Hüeb.) (Lepidoptera: Noctuidae) in Brazil: Elements for the environmental risk assessment of genetically modified cotton
Grantee:Samuel Martinelli
Support type: Scholarships in Brazil - Post-Doctorate