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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Soybean extracts enriched with free isoflavones promote nitric oxide synthesis and affect the proliferation of breast adenocarcinoma cells

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Author(s):
Ferreira, Pablo G. [1] ; Frungillo, Lucas [1] ; Rosso, Veridiana V. [2] ; Machado, Daisy [3] ; Mercadante, Adriana Z. [2] ; Ferreira, Carmen V. [3] ; Salgado, Ione [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencias Alimentos, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 23, n. 1, p. 86-93, JAN-FEB 2013.
Web of Science Citations: 0
Abstract

Although soybean isoflavones naturally accumulate in their conjugated forms, the beneficial effects on human health of soybean-containing foods have been credited to their aglycone forms. In the present study we analyzed the effects of a chemical agent, sodium nitroprusside (SNP), in eliciting the exudation of non-conjugated isoflavones from intact soybean seeds, embrionary axes and cotyledons. The isoflavones in the exudates were determined by high performance liquid chromatography and mass spectrometry. The effect of the exudates on the emission of nitric oxide (NO) and on the proliferation of breast carcinoma cells (MCF-7) was also evaluated. SNP elicitation increased the production of the aglycone forms dose- and time-dependently. Exudates of embrionary axes and cotyledons stimulated NO emission and showed biphasic effects on viability of MCF-7 cells. At lower concentrations both extracts presented proliferative effects (10-25%), and at higher concentrations inhibited (15%) cell proliferation. The biphasic effect might be due to the action of isoflavone aglycones in activating estrogen receptors which in turn stimulate the production of NO. Overall, the results suggest that soybean extracts enriched in isoflavone aglycones by elicitation with SNP could be exploited as a functional ingredient in the food industry. (AU)

FAPESP's process: 08/11636-5 - Nitric oxide signaling in plant defense response against pathogen attack: analysis of gene expression, production of secundary compounds and mitochondrial bioenergetics
Grantee:Ione Salgado
Support Opportunities: Regular Research Grants